PVRL4 antibody (Extracellular, N-Term)

Catalog No. ABIN7886645

The Rabbit Polyclonal anti-PVRL4 antibody (ABIN7886645) specifically detects PVRL4 in WB and FACS. The antibody is reactive with Mouse samples.

Quick Overview for PVRL4 antibody (Extracellular, N-Term) (ABIN7886645)

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PVRL4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-PVRL4 Antibody

Binding Specificity: AA 84-96, Extracellular, N-Term

Purpose: A Rabbit Polyclonal antibody to Nectin-4 (extracellular).

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - 12 out of 13 amino acid residues identical Human - 11 out of 13 amino acid residues identical

Characteristics: Anti-Nectin-4 (extracellular) Antibody (ABIN7043442, ABIN7044698 and ABIN7044699) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize Nectin-4 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KYGLHVNPAYEDR, corresponding to amino acid residues 84 - 96 of mouse Nectin-4

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236280)

Blocking Peptide: (ABIN7236280)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for PVRL4

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Alternative Name: NECTIN4

Background:

Synonyms: Nectin Cell Adhesion Molecule 4, Poliovirus Receptor-Related Protein 4, PVRL4, NECTIN4

Description: Nectin Cell Adhesion Molecule 4 (nectin-4), also known as Poliovirus Receptor-Related Protein 4 (PVRL4), is a Ca2 + -independent type 1 transmembrane protein that is a member of the immunoglobulin-like adhesion molecules protein family 1.All nectins (Nectin-1, Nectin-2, Nectin-3, and Nectin-4) share common structure features: an extracellular region of three Ig-like domains, a transmembrane region, and a cytoplasmic tail region 2.Nectin-4 interacts with afadin, a protein that has a role in cell-cell adhesion, and with nectin-1, but not with nectin-2 or 3 3.Nectin-4 was found to be overexpressed in several human cancers, including lung, ovarian, pancreatic, and breast cancer. It was also demonstrated that a soluble form of Nectin-4 is a potential diagnostic marker for several cancers and that knockdown of Nectin-4 inhibits tumor growth and reduces cell-cell contact in certain cancer types 4.Recently, an antibody-drug conjugate designed for the treatment of cancer expressing Nectin-4 was approved for use in bladder cancer patients.

Gene ID: 71740

NCBI Accession: NM_030916

UniProt: Q8R007

Pathways: Cell-Cell Junction Organization