KCNK1 antibody (Extracellular, P1 Loop)

Catalog No. ABIN7886920

This Rabbit Polyclonal antibody specifically detects KCNK1 in WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for KCNK1 antibody (Extracellular, P1 Loop) (ABIN7886920)

Target: KCNK1 (Potassium Channel Subfamily K Member 1 (KCNK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-KCNK1 Antibody

Binding Specificity: AA 53-69, Extracellular, P1 Loop

Purpose: A Rabbit Polyclonal Antibody to KCNK1 Channel

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,pig - identical

Characteristics: Anti-KCNK1 (extracellular) Antibody is directed against an epitope of the human K2P1.1 channel. Anti-KCNK1  (extracellular) Antibody (ABIN7043443, ABIN7045008 and ABIN7045009) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting KCNK1 in living cells. It has been designed to recognize KCNK1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: RQELRKLKRRFLEEHEC, corresponding to amino acid residues 53-69 of human KCNK1 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:300

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236284)

Blocking Peptide: (ABIN7236284)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for KCNK1

Target: KCNK1 (Potassium Channel Subfamily K Member 1 (KCNK1))

Alternative Name: KCNK1

Background:

Synonyms: Potassium channel subfamily K member 1, Inward rectifying K+ channel protein TWIK-1, K2P1.1, HOHO1, KCNO1

Description: KCNK1 (also named TWIK-1 or K2P1.1) is a member of the 2-pore (2P) domain K+ channels family that, currently, includes 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH , mechanical stretch, inhalation anesthetics, etc. but are insensitive to the classical K+ channel blockers.KCNK1 was the first of the K2P channels to be identified and as its original name indicates (Tandem of P domains in a Weak Inward rectifier K+ channel) the channel behaves as a weak inward rectifier when expressed in heterologous systems. The channel is ubiquitously expressed with the most prominent expression in the brain, kidney and heart.

Gene ID: 3775

NCBI Accession: NM_002245

UniProt: O00180