KCNK15 antibody (Extracellular)

Catalog No. ABIN7043445

Product Details anti-KCNK15 Antibody

Target: KCNK15 (Potassium Channel Subfamily K Member 15 (KCNK15))

Binding Specificity: AA 43-57, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNK15 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to KCNK15 Channel

Specificity: 1st extracellular loop

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for human samples

Predicted Reactivity: Mouse - identical

Characteristics: Anti-KCNK15 (TASK-5) (extracellular) Antibody is directed against an epitope of the rat KCNK15 channel. Anti-KCNK15 (TASK-5) (extracellular) Antibody (ABIN7043445, ABIN7045054 and ABIN7045055)) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize KCNK15 from rat and mouse samples. The antibody will unlikely recognize the channel from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RKRGEFRRKYRFSAD, corresponding to amino acid residues 43-57 of rat KCNK15 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-PC158

Blocking Peptide: BLP-PC158

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNK15

Target: KCNK15 (Potassium Channel Subfamily K Member 15 (KCNK15))

Alternative Name: KCNK15 (KCNK15 Products)

Background: Potassium channel subfamily K member 15, Acid-sensitive K+ channel protein TASK-5, TWIK-related acid-sensitive K+ channel 5, K2P15.1,TASK1-5 are members of the two-pore domain K+ channels. They have four transmembrane domains and assist in setting the resting membrane potential.TASK5 (K2P15.1, KCNK15) was identified using the TASK-3 sequence in a GenBank search. However, when expressed in COS-7 cells, it does not form a functional K+ current1.The channel is distributed in the brain, heart, lung, kidney, liver, pancreas, adrenal gland and placenta2.

Alternative names: KCNK15 (TASK-5), Potassium channel subfamily K member 15, Acid-sensitive K+ channel protein TASK-5, TWIK-related acid-sensitive K+ channel 5, K2P15.1

Gene ID: 156873

NCBI Accession: NM_022358

UniProt: Q8R5I0