KCNMA1 antibody (Intracellular)

Catalog No. ABIN7043494

Product Details anti-KCNMA1 Antibody

Target: KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))

Binding Specificity: AA 1184-1200, Intracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to KCNMA1 (KCa1.1) Channel

Specificity: Intracellular, C-terminal domain

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, human,bovine,chicken,dog - 16,17 amino acid residues identical

Characteristics: Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (ABIN7043494, ABIN7045002 and ABIN7045003) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize KCNMA1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)STANRPNRPKSRESRDK, corresponding to amino acid residues 1184-1200 of mouse KCNMA1 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNMA1

Target: KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))

Alternative Name: KCNMA1 (KCNMA1 Products)

Background: Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1,KCa1.1 (KCNMA1, BKCa, Maxi K+ or slo) is part of a structurally diverse group of K+ channels that are activated by an increase in intracellular Ca2+. KCa1.1 shows a large single channel conductance when recorded electrophysiologically and hence its name. It differs from the rest of the subfamily members in that it can be activated by both an increase in intracellular Ca2+ and by membrane depolarization. KCa1.1 is expressed in virtually all cell types where it causes hyperpolarization and helps to connect intracellular Ca2+ signaling pathways and membrane excitability.Indeed, KCa1.1 channels have a crucial role in smooth muscle contractility, neuronal spike shaping and neurotransmitter release.

Alternative names: KCNMA1 (KCa1.1), Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1

Gene ID: 16531

NCBI Accession: NM_002247

UniProt: Q08460

Pathways: Regulation of Hormone Metabolic Process, Sensory Perception of Sound