Nerve Growth Factor antibody (Pro-Domain)

Catalog No. ABIN7043556

The Rabbit Polyclonal anti-Nerve Growth Factor antibody (ABIN7043556) specifically detects Nerve Growth Factor in WB, IHC and IF. The antibody is reactive with Rat samples.

Quick Overview for Nerve Growth Factor antibody (Pro-Domain) (ABIN7043556)

Target: Nerve Growth Factor (NGF)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nerve Growth Factor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Nerve Growth Factor Antibody

Binding Specificity: AA 84-104, Pro-Domain

Purpose: A Rabbit Polyclonal Antibody to proNGF

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): The antibody is specific for proNGF, it does not crossreact with proBDNF, proNT-3 or mature NGF.

Predicted Reactivity: Mouse - identical, human - 16,21 amino acid residues identical

Characteristics: Anti-proNGF Antibody is directed against rat proNGF. Anti-proNGF Antibody (ABIN7043556, ABIN7044751 and ABIN7044752) can be used in western blot and immunohistochemical applications. The antibody specifically reacts with proNGF. It has been designed to recognize proNGF from mouse, rat and human samples. \nRelated products for neutralizing proNGF activity: \nMouse NGF/proNGF Neutralizing Antibody can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor)

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IP|IHC

Negative Control: (ABIN7236601)

Blocking Peptide: (ABIN7236601)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF

Background:

Synonyms: Nerve growth factor precursor

Description: Neurotrophins are synthesized as pro-forms that can be cleaved either intracellularly to release mature, secreted ligands, or extracellularly by various proteases such as plasmin, furin, PC1/3, PC7, and PACE 4.1,2,5 The immature precursor has a prodomain of 103 amino acids, which was thought to have a role in the folding and sorting of the mature NGF into the various secretion pathways. It was recently reported that proNGF, binds p75NTR receptor preferentially over TrkA, and this selective binding of proNGF to p75NTR leads to apoptotic death of cells that express both TrkA and p75NTR. However, mature NGF binds and activates both receptors, with resulting promotion of cell survival due to the TrkA-mediated survival signal overriding p75NTR -mediated apoptotic signal.3,4 Since pro- and mature neurotrophins seem to elicit opposite functional effects, by differential interactions with Trks and p75NTR receptors, extracellular cleavage represents a new way to control the synaptic functions of neurotrophins.It was demonstrated that proNGF from injured spinal cord extracts, is active and induce apoptosis among oligodendrocytes, and apoptosis can be blocked by a proNGF-specific antibody.6 Finally, proNGF was demonstrated as the predominant form in mouse, rat, and human brain tissue, thyroid, hippocampus, thus suggesting a role for proNGF in vivo.5,7,8

Gene ID: 310738

NCBI Accession: NM_002506

UniProt: P25427

Pathways: Regulation of Cell Size