Pro BDNF antibody (Pro-Domain)

Catalog No. ABIN7043557

Product Details anti-Pro BDNF Antibody

Target: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Binding Specificity: AA 72-88, Pro-Domain

Reactivity: Human, Mouse

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Pro BDNF antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity (Details): The antibody is specific for proBDNF, it does not crossreact with proNGF, proNT-3 or mature BDNF.

Characteristics: Guinea pig Anti-proBDNF Antibody is directed against the prodomain region of human proBDNF. Guinea pig Anti-proBDNF Antibody (#), raised in guinea pig, can be used in western and immunohistochemistry applications. In addition, the antibody is an excellent tool for easy multiplex staining studies. It has been designed to recognize proBDNF from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-proBDNF Antibody (ABIN7043558, ABIN7044753 and ABIN7044754)) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Pro BDNF

Target: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Alternative Name: proBDNF (proBDNF Products)

Background: Alternative names: proBDNF, Brain-derived neurotrophic factor precursor 

Gene ID: 627

NCBI Accession: NM_170735

UniProt: P23560