Pro BDNF antibody (AA 72-88) (Atto 488)

Catalog No. ABIN7043559

Product Details anti-Pro BDNF Antibody (Atto 488)

Target: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Binding Specificity: AA 72-88

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pro BDNF antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: A Rabbit Polyclonal Antibody to proBDNF Conjugated to the Fluorescent Dye ATTO-488

Specificity: Pro-domain of the BDNF protein

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 16,17 amino acid residues identical

Characteristics: Anti-proBDNF Antibody (ABIN7043558, ABIN7044753 and ABIN7044754) is a highly specific antibody directed against the prodomain region of human proBDNF. The antibody can be used in western blot, immunoprecipitation, and immunohistochemistry applications. It has been designed to recognize proBDNF from human, mouse, and rat samples. The antibody does not cross react with mature BDNF, pro- and mature NGF or mature . \nAnti-proBDNF-ATTO Fluor-488 Antibody (ABIN7043559) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-proBDNF-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582041)

Blocking Peptide: (ABIN7236605)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Pro BDNF

Target: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Alternative Name: proBDNF (proBDNF Products)

Background: Brain-derived neurotrophic factor precursor,Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.Until recently, the functional role of the neurotrophin prodomains were thought to include assistance in the correct folding of the mature protein and the sorting of the neurotrophins into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions.The functional importance of the prodomain of BDNF was recently demonstrated in a study showing that a polymorphism that replaces valine for methionine at position 66 of the prodomain, is associated with memory defects and abnormal hippocampal function in humans. Another recent study showed that the regulated extracellular cleavage of proBDNF to mature BDNF by plasmin is necessary for establishing late-phase long-term potentiation (L-LTP), a process that involves long-lasting changes in the structure and function of hippocampal synapses. Finally, proBDNF was shown to be decreased in the brains of patients suffering from Alzheimer's disease.Mature BDNF binds to the specific tyrosine kinase receptor TrkB and to p75NTR, a member of the TNF receptor superfamily. proBDNF can similarly bind both receptors although it appears to have a greater affinity for the p75NTR receptor.

Alternative names: proBDNF, Brain-derived neurotrophic factor precursor

Gene ID: 627

NCBI Accession: NM_170735

UniProt: P23560