Prokineticin Receptor 1 antibody (Extracellular)

Catalog No. ABIN7043560

This Rabbit Polyclonal antibody specifically detects Prokineticin Receptor 1 in WB, IHC, FACS, IF and LCI. It exhibits reactivity toward Rat.

Quick Overview for Prokineticin Receptor 1 antibody (Extracellular) (ABIN7043560)

Target: Prokineticin Receptor 1 (PROKR1)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prokineticin Receptor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Product Details anti-Prokineticin Receptor 1 Antibody

Binding Specificity: AA 10-24, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Prokineticin Receptor 1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Unlikely to recognize human samples.

Predicted Reactivity: Mouse - 12,15 amino acid residues identical

Characteristics: Anti-Prokineticin Receptor 1 (extracellular) Antibody is directed against an epitope located at the extracellular N-terminal domain of the rat Prokineticin receptor 1. The epitope is specific for PROKR1 and will not cross-react with the closely related PROKR2. Anti-Prokineticin Receptor 1 (extracellular) Antibody (ABIN7043560 and ABIN7045110) can be used in western blot, immunohistochemistry and flow cytometry applications, and recognizes PROKR1 from rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ENTTNTFTDFFSARD, corresponding to amino acid residues 10-24 of rat PROKR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: (ABIN7236609)

Blocking Peptide: (ABIN7236609)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Prokineticin Receptor 1

Target: Prokineticin Receptor 1 (PROKR1)

Alternative Name: PROKR1

Background: PROKR1, PKR1, G-protein coupled receptor 73, Gpr73, Gpr73a, G-protein coupled receptor ZAQ,The Prokineticins (PK1 and PK2) are a pair of cysteine-rich secreted peptides with broad physiological functions including gastrointestinal motility, angiogenesis, hematopoiesis and circadian rhythms regulation.The biological effects of PK1 and PK2 are mediated by two highly homologous receptors termed Prokineticin receptor 1 (PKR1) and Prokineticin receptor 2 (PKR2) that belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily. Both PK ligands activate the two PK receptors with similar potency.1PKR1 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization. In addition, activation of the mitogen-activated protein kinase (MAPK) pathways has also been described.1PKR1 is broadly distributed throughout peripheral tissues including the intestinal tract, testis, uterus, lung and peripheral blood leukocytes. In addition, the receptor is expressed in the brain, particularly in olfactory regions, as well as in dorsal root ganglion (DRG) neurons.The physiological function of PKR1 is still being elucidated, however, evidence suggest that the receptor has an important role in pain perception through its ability to stimulate the TRPV1 ion channel in DRG neurons.2In addition, a role for PKR1 in the control of the inflammatory response through regulation of macrophage chemotaxis and cytokine production has also been demonstrated.3

Alternative names: Prokineticin Receptor 1, PROKR1, PKR1, G-protein coupled receptor 73, Gpr73, Gpr73a, G-protein coupled receptor ZAQ

Gene ID: 192648

NCBI Accession: NM_138964

UniProt: Q8R416

Pathways: Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma