Prokineticin Receptor 2 antibody (Extracellular)

Catalog No. ABIN7043561

Product Details anti-Prokineticin Receptor 2 Antibody

Target: Prokineticin Receptor 2 (PROKR2)

Binding Specificity: AA 3-15, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prokineticin Receptor 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Prokineticin Receptor 2

Specificity: Extracellular, N-terminus

Predicted Reactivity: Mouse - 11,13 amino acid residues identical, human - 9

Characteristics: Anti-Prokineticin Receptor 2 (extracellular) Antibody is directed against an epitope located at the extracellular N-terminal domain of the rat Prokineticin receptor 2. The epitope is specific for PROKR2 and will not cross-react with the closely related PROKR1. Anti-Prokineticin Receptor 2 (extracellular) Antibody (ABIN7043561 and ABIN7045111)) can be used in western blot and immunohistochemical applications. It has been designed to recognize PROKR2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DQNGNTSFAPDLN, corresponding to amino acid residues 3-15 of rat PROKR2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-PR042

Blocking Peptide: BLP-PR042

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Prokineticin Receptor 2

Target: Prokineticin Receptor 2 (PROKR2)

Alternative Name: PROKR2 (PROKR2 Products)

Background: PROKR2, PKR2, G-protein coupled receptor 73-like 1, Gpr73l1, Gpr73b, G-protein coupled receptor I5E,The Prokineticins (PK1 and PK2) are a pair of cysteine-rich secreted peptides with broad physiological functions including gastrointestinal motility, angiogenesis, hematopoiesis and circadian rhythms regulation.The biological effects of PK1 and PK2 are mediated by two highly homologous receptors termed Prokineticin receptor 1 (PKR1) and Prokineticin receptor 2 (PKR2) that belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily. Both PK ligands activate the two PK receptors with similar potency.1PKR2 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization.The distribution of PKR2 is relatively restricted with high expression levels in the brain, spinal cord and dorsal root ganglions, in organs of the reproductive system and in endocrine tissues such as the thyroid, pituitary and adrenal glands.1The PK2 ligand has been shown to be involved in the regulation of circadian rhythms of physiological and behavioral processes in mammals, probably through signaling via PKR2 which is highly expressed in the suprachiasmatic nucleus (SCN), an area of the brain that controls circadian rhythm processes.2In addition, loss-of-function mutations in the PKR2 gene have been associated with Kallmann Syndrome, a condition characterized by idiopathic hypogonadotropic hypogonadism (IHH) in combination with anosmia, a compromised sense of smell.3

Alternative names: Prokineticin Receptor 2, PROKR2, PKR2, G-protein coupled receptor 73-like 1, Gpr73l1, Gpr73b, G-protein coupled receptor I5E

Gene ID: 192649

NCBI Accession: NM_144773

UniProt: Q8R415

Pathways: Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma