Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 1039-1054), (Intracellular) antibody

RIMS1 Reactivity: Rat WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7043597
  • Target See all Regulating Synaptic Membrane Exocytosis 1 (RIMS1) Antibodies
    Regulating Synaptic Membrane Exocytosis 1 (RIMS1)
    Binding Specificity
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1039-1054, Intracellular
    Reactivity
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    Rat
    Host
    • 5
    • 1
    Rabbit
    Clonality
    • 6
    Polyclonal
    Conjugate
    • 6
    Un-conjugated
    Application
    • 6
    • 3
    • 3
    • 1
    Western Blotting (WB)
    Purpose
    A Rabbit Polyclonal Antibody to RIM1
    Specificity
    Intracellular
    Cross-Reactivity
    Mouse, Rat
    Predicted Reactivity
    human - 14,16 amino acid residues identical,Mouse - 15
    Characteristics
    Anti-RIM1 Antibody is directed against an epitope of rat RIM1. Anti-RIM1 Antibody (ABIN7043597, ABIN7044528 and ABIN7044529) can be used in western blot analysis. It has been designed to recognize RIM1 from mouse, rat and human samples.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)RAKRGRSAESLHMTSE, corresponding to amino acid residues 1039-1054 of rat RIM1

    Isotype
    IgG
    Top Product
    Discover our top product RIMS1 Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: 1:200

    Comment

    Negative Control: (ABIN7236714)

    Blocking Peptide: (ABIN7236714)

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    Regulating Synaptic Membrane Exocytosis 1 (RIMS1)
    Alternative Name
    RIMS1 (RIMS1 Products)
    Background
    Regulating synaptic membrane exocytosis 1, Rab-3-interacting molecule 1, RIMS1,RIM1 is a member of the RIM superfamily of proteins (Rim1-4). These genes express seven known RIM isoforms: RIM1α, 1β, RIM2α, 2β, 2γ, and RIM3γ and RIM4γ which serve as scaffolding proteins in presynaptic nerve terminals by interacting with several other protein components localized at the active zone, including Munc13, ELKS (or CAST), liprins and voltage-gated Ca2+ channels (VGCCs). RIM1 directly binds to the C-terminal regions of the α1 subunit of N- and P/Q-type calcium channels, and can also interact with the β auxiliary subunit of P/Q-type calcium channels1-3.RIM proteins were found to be required for normal Ca2+ triggering of exocytosis. This interaction with Ca2+ channel significantly slows channel inactivation resulting in increased Ca2+ influx during action potentials.Deficiency of RIM1 decreases presynaptic Ca2+ channel density. RIM proteins are involved in neurotransmitter release and contribute to long- and short-term synaptic plasticity.

    Alternative names: RIM1, Regulating synaptic membrane exocytosis 1, Rab-3-interacting molecule 1, RIMS1
    Gene ID
    84556
    NCBI Accession
    NM_014989
    UniProt
    Q9JIR4
    Pathways
    Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
You are here:
Support