Septin 7 antibody (Intracellular)

Catalog No. ABIN7043614

This Rabbit Polyclonal antibody specifically detects Septin 7 in WB. It exhibits reactivity toward Rat.

Quick Overview for Septin 7 antibody (Intracellular) (ABIN7043614)

Target: Septin 7 (SEPT7)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Septin 7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Septin 7 Antibody

Binding Specificity: AA 83-96, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Septin-7

Specificity: Intracellular

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Septin-7 Antibody (ABIN7043614, ABIN7044544 and ABIN7044545) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Septin-7 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide (C)HRIKKTVQVEQSK, corresponding to amino acid residues 83-96 of rat Septin-7

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236776)

Blocking Peptide: (ABIN7236776)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Septin 7

Target: Septin 7 (SEPT7)

Alternative Name: SEPTIN7

Background: Sept7, CDC10, SEPT7A, NBLA02942, CDC3,Septin 7 is a highly conserved GTP-binding filamentous protein. Septins are important for the organization of the actin cytoskeleton. Septin7 also plays an important role in cytokinesis, and membrane dynamics1,2.Septins were first discovered in yeast and since, 13 septin genes have been identified in mammals (SEPT1 to SEPT12 and SEPT14). The 13 gene-encoded proteins can be classified into four distinct subgroups based on their sequence homology of their domain structure2.Septins share a highly conserved structure composed of the central GTPase domain and N- and C-terminal domains of various lengths3. They self-assemble into higher-order structures, including filaments and rings in tri-, hexa-, or octameric complexes consisting of multiple septin polypeptides, which are typical for different cell types. The various septin isoforms seem to determine the function and regulation of the septin complex4.Septin-7 regulates Ca2+ entry through Orai1 by regulating the opening of the channel5.Dysregulation of septins leads to human diseases such as neurodegenerative and bleeding disorders. In non-dividing cells such as neuronal tissue and platelets septins have been associated with exocytosis1,2.

Alternative names: Sept7, CDC10, SEPT7A, NBLA02942, CDC3

Gene ID: 64551

NCBI Accession: NM_001788

UniProt: Q9WVC0