Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Serotonin Receptor 1A antibody (AA 251-266)

The Rabbit Polyclonal anti-Serotonin Receptor 1A antibody has been validated for WB, IHC and IF. It is suitable to detect Serotonin Receptor 1A in samples from Rat.
Catalog No. ABIN7043615

Quick Overview for Serotonin Receptor 1A antibody (AA 251-266) (ABIN7043615)

Target

See all Serotonin Receptor 1A (HTR1A) Antibodies
Serotonin Receptor 1A (HTR1A)

Reactivity

  • 50
  • 30
  • 30
  • 1
  • 1
Rat

Host

  • 42
  • 6
  • 2
Rabbit

Clonality

  • 45
  • 5
Polyclonal

Conjugate

  • 24
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Serotonin Receptor 1A antibody is un-conjugated

Application

  • 35
  • 17
  • 13
  • 13
  • 13
  • 6
  • 5
  • 4
  • 4
  • 4
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
  • Binding Specificity

    • 15
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 251-266

    Purpose

    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1A

    Specificity

    3rd intracellular loop

    Cross-Reactivity

    Mouse, Rat

    Cross-Reactivity (Details)

    Will not recognize human 5-hydroxytryptamine receptor 1A.

    Predicted Reactivity

    Mouse - identical

    Characteristics

    Anti-5HT1A Receptor (HTR1A) Antibody is directed against an intracellular epitope of the rat 5-Hydroxytryptamine receptor 1A. Anti-5HT1A Receptor (HTR1A) Antibody (ABIN7043615, ABIN7045278 and ABIN7045279) can be used for western blot and immunohistochemistry applications. It has been designed to recognize 5HT1A receptor from rat and mouse samples.

    Purification

    Affinity purified on immobilized antigen.

    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: KKSLNGQPGSGDWRRC, corresponding to amino acid residues 251-266 of rat 5-Hydroxytryptamine receptor 1A

    Isotype

    IgG
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

    Application Dilutions Western blot wb: 1:200

    Comment

    Cited Application: IP

    Negative Control: (ABIN7236780)

    Blocking Peptide: (ABIN7236780)

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    0.2 mL double distilled water (DDW).

    Concentration

    1 mg/mL

    Buffer

    PBS pH 7.4

    Storage

    4 °C,-20 °C

    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target

    Serotonin Receptor 1A (HTR1A)

    Alternative Name

    HTR1A

    Background

    5-Hydroxytryptamine receptor 1A, Serotonin receptor 1A,Serotonin (5-hydroxytryptamine, 5-HT1) is an important neurotransmitter in the central nervous system (CNS) that exerts its neuronal activity via 5-HT receptors.5-HT receptors are subdivided into seven families termed 5-HT1 through 5-HT7.1 The 5-HT receptor families belong to the G-protein coupled receptor superfamily with the exception of 5-HT3 receptor class which belongs to the ligand-gated ion channels.2The 5-HT1 receptor class comprises of five different receptors (5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E and 5-HT1F) which share 41-63 % overall sequence identity and couple preferentially to Gi/Go to inhibit cAMP formation.2The 5-HT1A receptor is the most widespread of all the 5-HT receptors. In the CNS, 5-HT1A receptors exist in the cerebral cortex, hippocampus, septum, amygdala and raphe nucelus in high densities, and was also demonstrated in a variety of tissues from various species.2,3 Activation of 5-HT1A receptor decreases blood pressure and heart rate, and is involved in learning, memory, relieving anxiety and depression.1,4

    Alternative names: 5HT1A Receptor (HTR1A), 5-Hydroxytryptamine receptor 1A, Serotonin receptor 1A

    Gene ID

    24473

    NCBI Accession

    NM_000524

    UniProt

    P19327

    Pathways

    JAK-STAT Signaling, Synaptic Membrane, Feeding Behaviour
You are here:
Chat with us!