Serotonin Receptor 1A antibody (3rd Intracellular Loop)

Catalog No. ABIN7043615

The Rabbit Polyclonal anti-Serotonin Receptor 1A antibody is suitable to detect Serotonin Receptor 1A in samples from Rat. It has been validated for WB, IHC and IF.

Quick Overview for Serotonin Receptor 1A antibody (3rd Intracellular Loop) (ABIN7043615)

Target: Serotonin Receptor 1A (HTR1A)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Serotonin Receptor 1A Antibody

Binding Specificity: 3rd Intracellular Loop, AA 251-266

Purpose: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1A

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Will not recognize human 5-hydroxytryptamine receptor 1A.

Predicted Reactivity: Mouse - identical

Characteristics: Anti-5HT1A Receptor (HTR1A) Antibody is directed against an intracellular epitope of the rat 5-Hydroxytryptamine receptor 1A. Anti-5HT1A Receptor (HTR1A) Antibody (ABIN7043615, ABIN7045278 and ABIN7045279) can be used for western blot and immunohistochemistry applications. It has been designed to recognize 5HT1A receptor from rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: KKSLNGQPGSGDWRRC, corresponding to amino acid residues 251-266 of rat 5-Hydroxytryptamine receptor 1A

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IP

Negative Control: (ABIN7236780)

Blocking Peptide: (ABIN7236780)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Serotonin Receptor 1A

Target: Serotonin Receptor 1A (HTR1A)

Alternative Name: HTR1A

Background:

Synonyms: 5-Hydroxytryptamine receptor 1A, Serotonin receptor 1A

Description: Serotonin (5-hydroxytryptamine, 5-HT1) is an important neurotransmitter in the central nervous system (CNS) that exerts its neuronal activity via 5-HT receptors.5-HT receptors are subdivided into seven families termed 5-HT1 through 5-HT7.1 The 5-HT receptor families belong to the G-protein coupled receptor superfamily with the exception of 5-HT3 receptor class which belongs to the ligand-gated ion channels.2The 5-HT1 receptor class comprises of five different receptors (5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E and 5-HT1F) which share 41-63 % overall sequence identity and couple preferentially to Gi/Go to inhibit cAMP formation.2The 5-HT1A receptor is the most widespread of all the 5-HT receptors. In the CNS, 5-HT1A receptors exist in the cerebral cortex, hippocampus, septum, amygdala and raphe nucelus in high densities, and was also demonstrated in a variety of tissues from various species.2,3 Activation of 5-HT1A receptor decreases blood pressure and heart rate, and is involved in learning, memory, relieving anxiety and depression.1,4

Gene ID: 24473

NCBI Accession: NM_000524

UniProt: P19327

Pathways: JAK-STAT Signaling, Synaptic Membrane, Feeding Behaviour