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5HT1B Receptor antibody (Extracellular) (Atto 488)

This Rabbit Polyclonal antibody specifically detects 5HT1B Receptor in IHC, IF, FACS and LCI. It exhibits reactivity toward Human.
Catalog No. ABIN7043617

Quick Overview for 5HT1B Receptor antibody (Extracellular) (Atto 488) (ABIN7043617)

Target

See all 5HT1B Receptor (HTR1B) Antibodies
5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))

Reactivity

  • 35
  • 14
  • 14
  • 1
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  • 1
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  • 1
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Human

Host

  • 33
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Rabbit

Clonality

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Polyclonal

Conjugate

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This 5HT1B Receptor antibody is conjugated to Atto 488

Application

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  • 3
  • 3
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  • 1
Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)
  • Binding Specificity

    • 7
    • 7
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    • 1
    • 1
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    AA 33-49, Extracellular

    Purpose

    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1B Conjugated to the Fluorescent Dye ATTO-488

    Specificity

    Extracellular, N-terminus

    Cross-Reactivity

    Human, Mouse, Rat

    Predicted Reactivity

    Rat - 15,17 amino acid residues identical, mouse - 14

    Characteristics

    Anti-5HT1B Receptor (HTR1B) (extracellular) Antibody (ABIN7043616, ABIN7045280 and ABIN7045281) is a highly specific antibody directed against an epitope of the human 5-Hydroxytryptamine receptor 1B. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize 5HT1B receptor from human, rat, and mouse samples. \nAnti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody (ABIN7043617) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The label is analogous to the well-known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

    Purification

    Affinity purified on immobilized antigen.

    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: CSAKDYIYQDSISLPWK, corresponding to amino acid residues 33-49 of human 5-Hydroxytryptamine receptor 1B

    Isotype

    IgG
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:80

    Application Dilutions Western blot wb: N/A

    Comment

    Negative Control: (ABIN7582041)

    Blocking Peptide: (ABIN7236784)

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

    Concentration

    1 mg/mL

    Buffer

    PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target

    5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))

    Alternative Name

    HTR1B

    Background

    5-Hydroxytryptamine receptor 1B, Serotonin receptor 1B, Serotonin 1D beta receptor, 5HT1D-beta, HTR1DB, HTR1D2,Serotonin (5-hydroxytryptamine, 5-HT) plays unequivocally a most important role in the normal physiology amongst higher eukaryotes, as do most neurotransmitters. The physiological roles of serotonin and its receptors range from appetite, regulation of the circadian rhythm, sexual behavior, memory and nociception. Serotonin is also involved in migraine and some psychiatric diseases such as depression anxiety and aggression1. Serotonin and its receptors also have an influence on the cardiovascular, pulmonary, gastrointestinal (GI) and genitourinary systems2.To date, some fifteen serotonin receptors have been cloned and grouped into seven families (5-HT1-5-HT7) based on their signaling mechanisms. The 5-HT1 receptor class comprises 5 different receptors with ranging homology. Apart from 5-HT3, all serotonin receptors belong to the G-protein coupled receptor superfamily and therefore span the membrane seven times3.The 5-hydroxytryptamine receptor 1B (5-HT1B) is involved in several physiological behaviors and psychiatric diseases, including locomotor activity, drug abuse reinforcement, migraine, anxiety states and aggressive behaviors1.Pharmacological studies suggest that 5-HT1B is expressed by both serotonergic and non-serotonergic neurons. In the nervous system, this receptor is located in the axon terminal of the plasma membrane of unmyelinated axons. It is predominantly localized on pre-synaptic membranes. Signal transduction studies of the receptor indicate that this receptor is coupled negatively to adenylyl cyclase as its activation induces a decrease in adenylyl cyclase activity1.

    Alternative names: 5HT1B Receptor (HTR1B), 5-Hydroxytryptamine receptor 1B, Serotonin receptor 1B, Serotonin 1D beta receptor, 5HT1D-beta, HTR1DB, HTR1D2

    Gene ID

    3351

    NCBI Accession

    NM_000863

    UniProt

    P28222

    Pathways

    JAK-STAT Signaling, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, S100 Proteins
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