Serotonin Receptor 2B antibody (Extracellular) (Atto 488)

Catalog No. ABIN7043620

This Rabbit Polyclonal antibody specifically detects Serotonin Receptor 2B in IHC, FACS and LCI. It exhibits reactivity toward Rat.

Quick Overview for Serotonin Receptor 2B antibody (Extracellular) (Atto 488) (ABIN7043620)

Target: Serotonin Receptor 2B (HTR2B)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 2B antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-Serotonin Receptor 2B Antibody (Atto 488)

Binding Specificity: AA 6-20, Extracellular

Purpose: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2B Conjugated to the Fluorescent Dye ATTO-488

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical,human - 11,15 amino acid residues identical

Characteristics: Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody (ABIN7043619, ABIN7045288 and ABIN7045289) is a highly specific antibody directed against an epitope of the rat 5-B receptor. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect 5HT2B receptor in living cells. It has been designed to recognize 5-hydroxytryptamine receptor 2B from rat, mouse and human samples. \nAnti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody (ABIN7043620) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KMSEQSTISEHILQK, corresponding to amino acid residues 6-20 of rat 5-Hydroxytryptamine receptor 2B

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:80

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582041)

Blocking Peptide: (ABIN7236792)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Serotonin Receptor 2B

Target: Serotonin Receptor 2B (HTR2B)

Alternative Name: HTR2B

Background: 5-Hydroxytryptamine receptor 2B, Serotonin receptor 2B, 5-HT-2B, 5-HT2F, Stomach fundus serotonin receptor,5-HT is involved in a variety of physiological and biological processes. In the brain, 5-HT has been found to affect sleep, mood, appetite, anxiety, aggression, perception, pain, and cognition1. Signaling of 5-HT is mediated by receptors that are located on the cell membrane of neurons and most other cells in the body. The 5-HT2 family consists of three G-protein coupled receptors (GPCRs): 5-HT2A, 5-HT2B, and 5-HT2C2. Like other members, they are transmembrane proteins consisting of seven membrane-spanning α-helical segments with an extracellular N-terminus and an intracellular C-terminus. The binding of 5-HT to one of its receptors is thought to elicit a conformational change that activates associated heterotrimeric G proteins and recruits other downstream signaling/scaffolding molecules, such as GPCR kinases and β-arrestins.5-HT2B receptors have been found to be present in both rodent and human tissues, particularly in the cardiovascular system, gastrointestinal tract, bone, and central nervous system2. Only low expression levels are found in the brain and blood. The highest expression levels are found in the stomach fundus3.Over-expression of 5-HT2B receptors in hearts of transgenic mice results in cardiac hypertrophy and decreased ventricular function due to enhanced extracellular matrix (ECM) and remodeling. Likewise, genetic deletion of 5-HT2B receptors was shown to lead to ventricular dilation and incomplete cardiac development5.

Alternative names: 5HT2B Receptor (HTR2B), 5-Hydroxytryptamine receptor 2B, Serotonin receptor 2B, 5-HT-2B, 5-HT2F, Stomach fundus serotonin receptor

Gene ID: 28581

NCBI Accession: NM_000867

UniProt: P30994

Pathways: JAK-STAT Signaling, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Carbohydrate Metabolic Process