Serotonin Receptor 4 antibody (Extracellular)

Catalog No. ABIN7043624

Product Details anti-Serotonin Receptor 4 Antibody

Target: Serotonin Receptor 4 (HTR4)

Binding Specificity: AA 168-180, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - 12,13 amino acid residues identical, human - 11

Characteristics: Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (ABIN7043624, ABIN7045290 and ABIN7045291)) is a highly specific antibody directed against an epitope of the rat Serotonin receptor 4. The antibody can be used in western blot, immunocytochemistry, and live cell imaging applications.  It has been designed to recognize 5HT4 receptor from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DVIEKRKFNHNSN, corresponding to amino acid residues 168-180 of rat 5-Hydroxytryptamine receptor 4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP

Negative Control: BLP-SR036

Blocking Peptide: BLP-SR036

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Serotonin Receptor 4

Target: Serotonin Receptor 4 (HTR4)

Alternative Name: HTR4 (HTR4 Products)

Background: 5-Hydroxytryptamine receptor 4, Serotonin receptor 4,5-hydroxytryptamine receptor 4 (5-HT4 receptors) are a subclass among the large family of receptors mediating serotonin actions at central and peripheral targets. 5-HT4 receptor activation has been implicated in learning and memory, feeding control and stress response. 5-HT4 receptors are encoded by a complex gene which in humans, generates ten different splice variants1, 2.The 5-HT4 receptor, a member of the seven transmembrane spanning G-protein-coupled receptors (GPCRs), couples via Gs to increase cAMP production. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs), the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side3.Within the central nervous system, these receptors are expressed in limbic structures including: hypothalamus, nucleus accumbens, prefrontal cortex, amygdala and hippocampus, where they contribute to emotional and cognitive functions4.Low levels of 5-HT4 receptors are associated with numerous neuropsychiatric disorders including Alzheimer's disease, Huntington's disease, feeding disorders and stress-related disorders such as anxiety and depression5.

Alternative names: 5HT4 Receptor (HTR4), 5-Hydroxytryptamine receptor 4, Serotonin receptor 4

Gene ID: 25324

NCBI Accession: NM_000870

UniProt: Q62758

Pathways: JAK-STAT Signaling, cAMP Metabolic Process, S100 Proteins