NALCN antibody (Domain 3, Extracellular)

Catalog No. ABIN7887119

The Rabbit Polyclonal anti-NALCN antibody (ABIN7887119) specifically detects NALCN in WB, IF, IHC, IC and LCI. The antibody is reactive with Rat samples.

Quick Overview for NALCN antibody (Domain 3, Extracellular) (ABIN7887119)

Target: NALCN (Sodium Leak Channel, Non-Selective (NALCN))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NALCN antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunochromatography (IC), Live Cell Imaging (LCI)

Product Details anti-NALCN Antibody

Binding Specificity: AA 902-914, Domain 3, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Sodium Leak Channel Non-Selective Protein (NALCN)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-NALCN/VGCNL1 (extracellular) Antibody (ABIN7043658 and ABIN7045249) is a highly specific antibody directed against an epitope of rat Sodium leak channel non-selective protein. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NALCN from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)SMMFESPFRRVMH, corresponding to amino acid residues 902-914 of rat NALCN

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:50

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236886)

Blocking Peptide: (ABIN7236886)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for NALCN

Target: NALCN (Sodium Leak Channel, Non-Selective (NALCN))

Alternative Name: NALCN

Background:

Synonyms: Sodium leak channel non-selective protein, Voltage gated channel-like protein 1, Four domain-type voltage-gated ion channel alpha-1 subunit, CanIon, Rb21-channel

Description: The existence of resting K+ conductance has been known for some time. Only recently has the Nalcn (sodium leak channel non-selective protein) been found responsible for the resting Na+ conductance1.Nalcn is a member of the NaV and CaV channel family which have four homologous repeats of six transmembrane domains. One structural difference is that Nalcn is less positively charged in the voltage sensing segment1. The protein forms voltage-independent non-inactivating channel which is permeable to Na+, K+ and Ca2+ ions1,2.Evidence suggests that Nalcn is a non-redundant and thus essential channel since knock-out mice display disrupted respiratory rhythm and die within 24 hours of birth. In addition, isolated neurons from these mice have decreased excitability1.The channel is extensively expressed in the central nervous system1, heart, pituitary and adrenal glands and pancreatic islet cells2,3.In pancreatic islets, Nalcn is activated by acetylcholine via its physical association with M3 muscarinic receptor, and potentiates insulin secretion induced by glucose uptake2-4.

Gene ID: 266760

NCBI Accession: NM_052867

UniProt: Q6Q760