SLC1A5 antibody (C-Term, Intracellular)

Catalog No. ABIN7886778

This Rabbit Polyclonal antibody specifically detects SLC1A5 in WB, IHC and IF. It exhibits reactivity toward Mouse.

Quick Overview for SLC1A5 antibody (C-Term, Intracellular) (ABIN7886778)

Target: SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC1A5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SLC1A5 Antibody

Binding Specificity: AA 502-516, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to ASCT2 Transporter

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Will not recognize ASCT2 from human samples.

Predicted Reactivity: Rat - 14,15 amino acid residues identical

Characteristics: Anti-ASCT2 (SLC1A5) Antibody (ABIN7043668, ABIN7044823 and ABIN7044824) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry analysis. It has been designed to recognize SLC1A5 from mouse and rat samples. It will not recognize human ASCT2.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KMPSSEPELIQVKNE, corresponding to amino acid residues 502-516 of mouse SLC1A5

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236918)

Blocking Peptide: (ABIN7236918)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SLC1A5

Target: SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))

Alternative Name: SLC1A5

Background:

Synonyms: Alanine/serine/cysteine transporter 2, Neutral amino acid transporter B(0), ATB(0), ATBO, Sodium-dependent neutral amino acid transporter type 2, AAAT

Description: ASCT2 is an alanine-serine-cysteine amino acid transporter and solute linked carrier family 1 member A5 (SLC1A5). It functions as an essential high-affinity glutamine transporter. It also functions as a Na+-dependent transporter for alanine, serine and cysteine. The transport process is electroneutral which involves the Na+-coupled influx of glutamine to the Na+-coupled efflux of another substrate such as alanine, serine or cysteine. ASCT2 is expressed various peripheral tissues such as the lung and kidney1,2. ASCT2 has an important contribution to the homeostasis of amino acid metabolism within tumor cells3.ASCT2 seems to be up regulated in several cancerous tissues such as stomach, liver, kidney, breast, prostate, skin, colorectal, pancreatic, tongue and lung cancers. Studies have shown that ASCT2 is necessary for activating critical survival and cellular growth signaling cascades in cancer, which includes mTOR and ERK pathways. It has been shown that knockdown of ASCT2 in prostate cancer cell line xenograft models significantly inhibit tumor growth and metastasis in vivo. Thus, ASCT2 is a potential cancer therapeutic target1,2.

Gene ID: 20514

NCBI Accession: NM_001145144

UniProt: P51912

Pathways: Dicarboxylic Acid Transport, Warburg Effect