SLC17A8 antibody (Cytosolic)

Catalog No. ABIN7043680

Product Details anti-SLC17A8 Antibody

Target: SLC17A8 (Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter), Member 8 (SLC17A8))

Binding Specificity: AA 533-545, Cytosolic

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC17A8 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 3

Specificity: Cytoplasmic, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - 12, human - 10,13 amino acid residues identical

Characteristics: Anti-VGLUT3 Antibody is directed against an epitope of rat Vesicular glutamate transporter 3. Anti-VGLUT3 Antibody (ABIN7043680, ABIN7044372 and ABIN7044373) can be used in western blot and immunohistochemistry applications. It has been designed to recognize VGLUT3 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ELNHEAFVSPRKK, corresponding to amino acid residues 533-545 of rat VGLUT3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:500

Comment:

Negative Control: (ABIN7236956)

Blocking Peptide: (ABIN7236956)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC17A8

Target: SLC17A8 (Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter), Member 8 (SLC17A8))

Alternative Name: SLC17A8 (SLC17A8 Products)

Background: Vesicular glutamate transporter 3, SLC17A8,Central nervous system neurons have traditionally been thought to express exclusively membrane transporters and/or vesicular transporters for their transmitter. Three vesicular glutamate transporters (VGLUTs) have recently been cloned: BNPI/VGLUT1 (a brain-specific sodium dependent inorganic phosphate (Pi) transporter), and its homologs DNPI/VGLUT2 (differentiation-associated sodium-dependent Pi transporter) and VGLUT31. These transporters mediate glutamate uptake inside presynaptic vesicles and are anatomical and functional markers of glutamatergic excitatory transmission2.VGLUT 1-3 are very similar in structure and function, but are used by different neuronal populations. VGLUT3 is a glycosylated transmembrane protein with ten putative transmembrane domains (TMD I-X) and both amino- and carboxyl-termini located in the cytosol3.VGLUT3 is localized in a limited number of glutamatergic neurons in multiple brain regions: neocortex, hippocampus, olfactory bulb, hypothalamus, substania nigra and raphe nuclei. Additionally, VGLUT3 is expressed in a population of symmetrical synapses. It is detected in hippocampal and cortical GABAergic neurons, cholinergic neurons in the striatum and serotonergic neurons in the raphe nuclei4.Mice lacking VGLUT3 are congenitally deaf due to loss of glutamate release at the inner hair cell afferent synapse5. In addition loss of VGLUT3 specifically impairs mechanical pain sensation6.

Alternative names: VGLUT3, Vesicular glutamate transporter 3, SLC17A8

Gene ID: 266767

NCBI Accession: NM_139319

UniProt: Q7TSF2

Pathways: Sensory Perception of Sound, Dicarboxylic Acid Transport