SLC38A1 antibody (Cytosolic, N-Term)

Catalog No. ABIN7886803

This Rabbit Polyclonal antibody specifically detects SLC38A1 in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for SLC38A1 antibody (Cytosolic, N-Term) (ABIN7886803)

Target: SLC38A1 (Solute Carrier Family 38 Member 1 (SLC38A1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC38A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SLC38A1 Antibody

Binding Specificity: AA 41-57, Cytosolic, N-Term

Purpose: A Rabbit Polyclonal Antibody to SLC38A1 Transporter

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-SLC38A1 Antibody (ABIN7043722, ABIN7044857 and ABIN7044858) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize SNAT1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)SKFISDRESRRSLTNSH, corresponding to amino acid residues 41-57 of rat SLC38A1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:500

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237083)

Blocking Peptide: (ABIN7237083)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SLC38A1

Target: SLC38A1 (Solute Carrier Family 38 Member 1 (SLC38A1))

Alternative Name: SLC38A1

Background:

Synonyms: Sodium-coupled neutral amino acid transporter 1, Snat1, Amino acid transporter A1, rATA1, Glutamine transporter, N-system amino acid transporter 2, Ata1, Glnt, Sa2, Sat1

Description: SNAT1 (SLC38A1, Sodium-coupled neutral amino acid transporter 1) is a 486 amino acids system A type sodium symporter that catalyzes the electrogenic Na+-dependent uptake of glutamine, among other substrates. SNAT1 is thought to be responsible for glutamine uptake into neurons1.SNAT1 belongs to the β-family of solute carriers that includes three SLC families, SLC32, SLC36, and SLC38. SNAT1 is a member of the SLC38 family that includes 11 members, SNAT1 to SNAT11.SNAT1 and SNAT2 are expressed in neurons and in glial cells all over the central nervous system, with high expression in perivascular glial profiles and lower expression in astrocytes of the cerebral parenchyma. This supports a role in controlling the amino acid permeability at the blood-brain barrier2. SNAT1 also acts as a positive regulator of mTORC1 signaling pathway in neurons and plays an important pathological role in the mechanism underlying neuronal survival after ischemia3. It was also shown to be up-regulated in specific types of cancer4.The structure of SNAT proteins is predicted to have a 5+5 inverted repeat fold typical of the bacterial LeuT, with intracellular N-terminus and extracellular and C-terminal tail5.

Gene ID: 170567

NCBI Accession: NM_001077484

UniProt: Q9JM15