SLC6A3 antibody (Extracellular)

Catalog No. ABIN7043740

Product Details anti-SLC6A3 Antibody

Target: SLC6A3 (Solute Carrier Family 6 (Neurotransmitter Transporter, Dopamine), Member 3 (SLC6A3))

Binding Specificity: AA 191-205, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Dopamine Transporter (DAT)

Specificity: 2nd extracellular loop

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for use with human samples.

Predicted Reactivity: Mouse - 14,15 amino acid residues identical

Characteristics: Anti-Dopamine Transporter (DAT) (extracellular) Antibody (ABIN7043740, ABIN7044606 and ABIN7044607) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize DAT from mouse and rat samples. The antibody is unlikely to recognize DAT from human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DAHASNSSDGLGLND, corresponding to amino acids residues 191-205 of rat DAT

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237153)

Blocking Peptide: (ABIN7237153)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC6A3

Target: SLC6A3 (Solute Carrier Family 6 (Neurotransmitter Transporter, Dopamine), Member 3 (SLC6A3))

Alternative Name: SLC6A3 (SLC6A3 Products)

Background: Sodium-dependent dopamine transporter, DA transporter, SLC6A3,The Na+/Cl- transporter family SLC6, includes DAT, a monoamine transporter, important for regulating extracellular levels of dopamine. It does so by taking up dopamine from the synaptic cleft via the co-transport of Na+ and Cl- down their electrochemical gradients1. The removal of dopamine by DAT remains the most important means to control the extracellular lifetime of the neurotransmitter and notably, the ending of dopaminergic neurotransmission2. Transporters for serotonin, norepinephrine, GABA and glycine also belong to this family3.These receptors have 12 transmembrane spanning domains and intracellular N- and C-termini. DAT is also subject to post translational modifications such as phosphorylation, important for its regulation1. It also possesses a large extracellular domain which undergoes N-glycosylation, important for the proper targeting of the transporter to the plasma membrane1. DAT is expressed in dopaminergic cell bodies and terminals and can therefore serve as a marker for these neurons4. DAT is also expressed in the retina, gastrointestinal tract, lung, kidney, pancreas and lymphocytes2.DAT plays an important role in movement as well as reward, learning and memory5. Its malfunction, which leads to dopaminergic dysregulation, has been associated with ADHD, schizophrenia, as well as Parkinson's disease2. DAT is the main target for cocaine, amphetamine and methamphetamine psychostimulants, which mainly increase locomotor activities2.

Alternative names: Dopamine Transporter (DAT), Sodium-dependent dopamine transporter, DA transporter, SLC6A3

Gene ID: 24898

NCBI Accession: NM_001044

UniProt: P23977

Pathways: Dopaminergic Neurogenesis