SLC7A2 antibody (Extracellular)

Catalog No. ABIN7043748

The Rabbit Polyclonal anti-SLC7A2 antibody (ABIN7043748) specifically detects SLC7A2 in IHC, WB, FACS and LCI. The antibody is reactive with Rat samples.

Quick Overview for SLC7A2 antibody (Extracellular) (ABIN7043748)

Target: SLC7A2 (Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System), Member 2 (SLC7A2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC7A2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-SLC7A2 Antibody

Binding Specificity: AA 151-163, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Cationic Amino Acid Transporter 2 (SLC7A2)

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 12,13 amino acid residues identical

Characteristics: Anti-SLC7A2 (extracellular) Antibody (ABIN7043748, ABIN7044831 and ABIN7044832) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize Cationic amino acid transporter 2 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KTYFKMNYTGLAE, corresponding to amino acid residues 151-163 of rat SLC7A2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7237184)

Blocking Peptide: (ABIN7237184)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC7A2

Target: SLC7A2 (Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System), Member 2 (SLC7A2))

Alternative Name: SLC7A2

Background: Cationic amino acid transporter 2, CAT-2, Low affinity cationic amino acid transporter 2, ATRC2, Solute carrier family 7 member 2,The cationic amino acid transporter (CAT) family is comprised of four homologous membrane proteins: CAT-1, CAT-2, CAT-3 and CAT-4, encoded by the SLC7A1-4 genes, respectively. The major difference between these transporters is at the level of tissue specificity and regulation of their expression1,2.The CAT family is included in the Na+-independent transport system, (named system y+) that mediate L-arginine uptake in most tissues and cells3.CAT-2 (SLC7A2) is known to transport cationic amino acids such as L-arginine, L-ornithine, and L-lysine. It is considered to be the major arginine transporter in most cells and tissues. It can be expressed in two spliced variants, CAT-2A and CAT-2B. CAT-2A, is a low-affinity transporter, constitutively expressed in liver and muscle cells, whereas CAT-2B, is a high-affinity transporter, abundantly expressed in macrophages3.Macrophages isolated from mice deficient in CAT-2 show impaired ability to transport arginine intracellularly and to produce nitric oxide in response to endotoxin, thus CAT2 may also be important in regulating immune cell function in inflammation4.Studies have identified CAT2 as a regulator of fibrotic responses in the lung5.

Alternative names: SLC7A2, Cationic amino acid transporter 2, CAT-2, Low affinity cationic amino acid transporter 2, ATRC2, Solute carrier family 7 member 2

Gene ID: 64554

NCBI Accession: NM_003046

UniProt: B5D5N9