SLC9A2 antibody (Intracellular)

Catalog No. ABIN7043757

The Rabbit Polyclonal anti-SLC9A2 antibody (ABIN7043757) specifically detects SLC9A2 in WB, IHC and IF. The antibody is reactive with Rat samples.

Quick Overview for SLC9A2 antibody (Intracellular) (ABIN7043757)

Target: SLC9A2 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 2 (SLC9A2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC9A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SLC9A2 Antibody

Binding Specificity: AA 797-812, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 2 (NHE-2)

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 12,16 amino acid residues identical

Characteristics: Anti-Na+/H+ Exchanger 2 Antibody (ABIN7043757, ABIN7044867 and ABIN7044868) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RASEPGNRKGRLGNEK, corresponding to amino acid residues 797-812 of rat NHE-2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237207)

Blocking Peptide: (ABIN7237207)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC9A2

Target: SLC9A2 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 2 (SLC9A2))

Alternative Name: SLC9A2

Background: Sodium/hydrogen exchanger 2, SLC9A2,In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH . They have adopted various mechanisms in order to do so, one of which is via Na+/H+ exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification1.To date, nine genes have been identified in mammals, NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-terminal. The C-terminal domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-terminal part of the protein also undergoes posttranslational modification such as phosphorylation2. Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated1,3,4.Under physiological conditions, the Na+/H+ exchanger mediates the exchange of one extracellular Na+ ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of Na+ is not selective as it can also bind Li+ and H+ 1,5,6. K+ ions inhibit NHE-1 but have no effect on NHE-27. The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH . Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH , they are rapidly activated1,5,8.NHE-2 is detected in the intestine, kidney and parietal cells2,9-11. It is also detected in skeletal muscle and testis2,12.

Alternative names: Na+/H+ Exchanger 2 (NHE-2), Sodium/hydrogen exchanger 2, SLC9A2

Gene ID: 24783

NCBI Accession: NM_003048

UniProt: P48763

Pathways: Proton Transport