SSTR5 antibody (Extracellular)

Catalog No. ABIN7043767

Product Details anti-SSTR5 Antibody

Target: SSTR5 (Somatostatin Receptor 5 (SSTR5))

Binding Specificity: AA 2-15, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SSTR5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunochromatography (IC), Immunofluorescence (IF), Live Cell Imaging (LCI)

Purpose: A Polyclonal Antibody to Somatostatin Receptor Type 5

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 12,14 amino acid residues identical

Characteristics: Anti-Somatostatin Receptor Type 5 (extracellular) Antibody is directed against an epitope of rat SSTR5. Anti-Somatostatin Receptor Type 5 (extracellular) Antibody (ABIN7043767 and ABIN7045267)), can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize SSTR5 in human, mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CEPLSLASTPSWNAS, corresponding to amino acid residues 2-15 of rat SSTR5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-SR005

Blocking Peptide: BLP-SR005

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SSTR5

Target: SSTR5 (Somatostatin Receptor 5 (SSTR5))

Alternative Name: SSTR5 (SSTR5 Products)

Background: SSTR5, SS5R, Smstr5, SST5,Somatostatin (SST) is a small cyclic peptide that was first identified as a powerful inhibitor of the secretion of various hormones including growth hormone (GH), thyroid-stimulating hormone (TSH) and prolactin from the pituitary, as well as almost every major hormone from the intestinal tract. SST consists of two major bioactive forms, SST-14 and the N-terminus extended peptide SST-28, that can be produced by a wide variety of neuroendocrine, inflammatory and immune cells. In target cells, SST induces a variety of physiological functions that include neuromodulation, cell secretion, cell proliferation and smooth muscle contractility.SST acts on its multiple cell targets via a family of six receptors that originate from five genes: SSTR1, SSTR2a, SSTR2b, SSTRR3, SSTR4, SSTR5. The SSTRs are members of the G-protein coupled receptor superfamily and they modulate cell response via multiple second messenger systems such as inhibition of adenylate cyclase, modulation of conductance of ion channels and protein dephosphorylation.In contrast to SSTR1-4 that bind SST-14 and SST-28 with similar affinity, SSTR5 has a much greater affinity to SST-28 over SST-14.SSTRs are widely expressed in both the brain and peripheral tissues but with receptor subtype variations between the different cell types.Strong SSTR expression has been detected in a variety of human tumors including pancreatic, breast, lung and prostate cancers. In fact, SST has been shown to inhibit the growth of various normal and tumor cells. On this basis, several long lasting SST analogs have been developed and are being tested for use in several pathologies such as various cancers, acromegaly, immunoproliferative disorders, diabetic retinopathy, epilepsy and pain.SSTR5 is expressed in a variety of tissues and cells, including pancreas, colonic mucosa, small intestine, heart, and vascular smooth muscle cells.SSTR5 has been shown to mediate growth inhibition and cell proliferation, and to play an important role in cancer growth regulation as one of the most potent inhibitory receptors.

Alternative names: Somatostatin Receptor Type 5, SSTR5, SS5R, Smstr5, SST5

Gene ID: 25354

NCBI Accession: NM_001053

UniProt: P30938

Pathways: Carbohydrate Homeostasis