S1PR5 antibody (Extracellular)

Catalog No. ABIN7043777

Product Details anti-S1PR5 Antibody

Target: S1PR5 (Sphingosine-1-Phosphate Receptor 5 (S1PR5))

Binding Specificity: AA 24-39, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 5

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-S1PR5/EDG8 (extracellular) Antibody (ABIN7043777, ABIN7045276 and ABIN7045277)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize EDG8 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KLRGARYQPGAGLRAD, corresponding to amino acid residues 24-39 of mouse S1PR5 (EDG8)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-SR015

Blocking Peptide: BLP-SR015

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for S1PR5

Target: S1PR5 (Sphingosine-1-Phosphate Receptor 5 (S1PR5))

Alternative Name: S1PR5 (S1PR5 Products)

Background: S1P receptor 5, S1P5, Endothelial differentiation G-protein-coupled receptor 8, Lysophospholipid receptor B4, Sphingosine 1-phosphate receptor Edg-8, S1P receptor Edg-8,Sphingosine-1-phosphate receptor 5 also known as S1PR5 (EDG8) is a G protein-coupled receptor which binds the lipid signaling molecule sphingosine 1-phosphate (S1P).Lysophospholipids encompass a broad range of small, membrane-derived phospholipids that act as extracellular signals, mediated through specific receptors1. S1PR5 is a high-affinity cell surface S1P receptor, a member of the S1PR family. Like all GPCRs it has the classical structure of seven transmembrane domains, an extracellular N-terminus and a cytosolic C-terminal1.The signaling pathways triggered by S1P/S1P binding play a critical regulatory role in many pathophysiological processes, including skeletal muscle and nervous system degeneration, inflammation, cancer, and autoimmune disorders and hence this interaction has important implications for clinical applications and for personalized medicine2,3. Targeting S1P receptors and their downstream signaling cascades is considered as a potential treatment for cancer and various autoimmune diseases4.

Alternative names: S1P receptor 5, S1P5, Endothelial differentiation G-protein-coupled receptor 8, Lysophospholipid receptor B4, Sphingosine 1-phosphate receptor Edg-8, S1P receptor Edg-8

Gene ID: 94226

NCBI Accession: NM_030760

UniProt: Q91X56