STOML1 antibody (Extracellular)

Catalog No. ABIN7043778

Product Details anti-STOML1 Antibody

Target: STOML1 (Stomatin (EPB72)-Like 1 (STOML1))

Binding Specificity: AA 4-17, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STOML1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Stomatin-Like Protein 1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-Stomatin-like Protein 1 (extracellular) Antibody (ABIN7043778, ABIN7044516 and ABIN7044517)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize STOML1 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RSGYRALPLGDFDR, corresponding to amino acid residues 4-17 of mouse STOML1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:500-1:800

Comment:

Negative Control: BLP-IP001

Blocking Peptide: BLP-IP001

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for STOML1

Target: STOML1 (Stomatin (EPB72)-Like 1 (STOML1))

Alternative Name: STOML1 (STOML1 Products)

Background: STOML1, SLP-1, STORP, Slipin-1, hUNC-24,The Stomatin-like protein family contains five members and are characterized by the presence of a structurally conserved core called the stomatin-domain. This stomatin-domain is further related to a domain found in members of the prohibitin, flotillin and HflK/HflC protein families and has accordingly also been called the SPFH domain.All mammalian stomatin proteins are integral membrane protein with a single, relatively short, hydrophobic membrane insertion domain, followed by the core stomatin-domain. A single conserved proline residue (known to be a potent helix breaker) in the hydrophobic region is required for the formation of the protein's hairpin structure. The N-terminal domain is extracellular. Stomatin-like protein 1 (STOML1) has a typical stomatin-domain at the N-terminus and an additional sterol carrier protein-2 (SCP-2) domain at the C-terminus. STOML-1 is targeted to the late endosomal compartment where it interacts with stomatin. STOML-1 is predominantly expressed in the brain, in a subpopulation of sensory neurons in the dorsal root ganglia. Much lower levels are detected in other tissues1.Following anoxia, STOML-1 expression is up-regulated by the oxidative response factor SKN-1/Nrf. SKN-1/Nrf is required for both rapid mitochondrial refusion and rapid behavioral recovery during reoxygenation suggesting that STOML-1 helps facilitate mitochondrial dynamics following anoxia2.STOML-1 also modulates the proton-sensitive members of the acid-sensing ion channel (ASIC) family. STOML-1 strongly inhibits ASIC1a and accelerates the inactivation time constant of ASIC3, but has no effect on the splice variant ASIC1b3.

Alternative names: Stomatin-like Protein 1, STOML1, SLP-1, STORP, Slipin-1, hUNC-24

Gene ID: 69106

NCBI Accession: NM_004809

UniProt: Q8CI66