Stim2 antibody (Intracellular)

Catalog No. ABIN7043782

Product Details anti-Stim2 Antibody

Target: Stim2 (Stromal Interaction Molecule 2 (Stim2))

Binding Specificity: AA 583-597, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Stim2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to STIM2

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,bovine - identical

Characteristics: Anti-STIM2 Antibody (ABIN7043782, ABIN7044028 and ABIN7044029)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize STIM2 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: SAEKQWEVPDTASEC, corresponding to amino acid residues 583-597 of human STIM2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|ICC

Negative Control: BLP-CC064

Blocking Peptide: BLP-CC064

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Stim2

Target: Stim2 (Stromal Interaction Molecule 2 (Stim2))

Alternative Name: STIM2 (Stim2 Products)

Background: Stromal interaction molecule 2,Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become the focus of intense research.Recently, several key players of the store-operated complex have been identified: the Orai family consists of three members: Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4While STIM1 appears to be localized intracellularly at the ER membrane and at a much lower extent on the cell surface of several cell types, STIM2 appears to be localized solely at the ER membrane.5 The function of STIM2 still remains elusive and several models have been suggested.6-8

Alternative names: STIM2, Stromal interaction molecule 2

Gene ID: 57620

NCBI Accession: NM_020860

UniProt: Q9P246

Pathways: TCR Signaling, BCR Signaling