SUCNR1 antibody (3rd Extracellular Loop)

Catalog No. ABIN7887167

The Rabbit Polyclonal anti-SUCNR1 antibody is suitable to detect SUCNR1 in samples from Rat. It has been validated for WB, IHC, FACS and LCI.

Quick Overview for SUCNR1 antibody (3rd Extracellular Loop) (ABIN7887167)

Target: SUCNR1 (Succinate Receptor 1 (SUCNR1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUCNR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-SUCNR1 Antibody

Binding Specificity: 3rd Extracellular Loop, AA 248-262

Purpose: A Rabbit Polyclonal Antibody to Succinate Receptor 1

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 15 amino acid residues identical,Mouse - 14, human - 12

Characteristics: Anti-GPR91 (SUCNR1) (extracellular) Antibody (ABIN7043783, ABIN7045308 and ABIN7045309) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize GPR91 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RNVRIASRLDSWPQG, corresponding to amino acid residues 248-262 of rat GPR91/Succinate Receptor 1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 mg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IFC

Negative Control: (ABIN7237300)

Blocking Peptide: (ABIN7237300)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SUCNR1

Target: SUCNR1 (Succinate Receptor 1 (SUCNR1))

Alternative Name: SUCNR1

Background:

Synonyms: G-protein coupled receptor 91, Succinate receptor 1

Description: GPR91 (Succinate Receptor 1), a member of the GPCR superfamily, is specifically activated by extracellular succinate outside the tricarboxylic acid cycle, resulting in activation of paracrine and endocrine effectors. Activation of GPR91 is associated with several diseases, including hypertension and diabetes1,2.GPR91 was shown to regulate normal retinal vascularization, proliferative ischemic retinopathy and cortical revascularization post-ischemia. Activation of GPR91 influences different tissues such as kidney, heart, retina, white adipose tissue, and liver.Through the activation of GPR91, succinate affects motility, migration, and growth of dendritic cells. In addition, the receptor directly promotes chemotaxis and potentiates activation initiated by Toll-like receptor agonists in dendritic cells1-3.In the heart GPR91 mediates succinate-induced cardiomyocyte death. It was demonstrated that upholding levels of serum succinate can cause cardiac hypertrophy through the activation of the GPR911,2.

Gene ID: 408199

NCBI Accession: NM_033050

UniProt: Q6IYF9