SNAP25 antibody (Cytosolic)

Catalog No. ABIN7043792

The Rabbit Polyclonal anti-SNAP25 antibody has been validated for WB, IHC and IF. It is suitable to detect SNAP25 in samples from Rat.

Quick Overview for SNAP25 antibody (Cytosolic) (ABIN7043792)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNAP25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SNAP25 Antibody

Binding Specificity: Cytosolic

Purpose: A Rabbit Polyclonal Antibody to SNAP-25

Specificity: Cytoplasmic

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,mouse - identical

Characteristics: Anti- Antibody is directed against rat . Anti- Antibody (ABIN7043792 and ABIN7044647) can be used in western blot and immunohistochemical applications. It has been designed to recognize from rat, mouse and human samples.

Purification: The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

Immunogen:

Immunogen: GST fusion protein

Immunogen Sequence: GST fusion protein with the sequence MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARV VDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG, corresponding to the full-length of rat SNAP-25

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 3 μg fusion protein per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:1000

Comment:

Cited Application: IHC

Negative Control: (ABIN7237327)

Blocking Peptide: (ABIN7237327)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: SNAP25

Background: Synaptosomal-associated protein 25 kD,SNAP-25 (synaptosomal-associated 25kD protein) is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.SNAP-25, unlike most SNAREs, lacks a membrane-spanning region, but is attached to the cytoplasmic side of the plasma membrane by posttranslational modifications such as palmitoylation or farnesylation1,2.SNAP-25 has been extensively studied for its role on neuronal and neuroendocrine cell exocytosis where it functions as one of the plasma membrane protein t-SNARE, which together with the vesicular v-SNARE protein VAMP and another t-SNARE such as Syntaxin 1, forms a trimeric, four-helical complex, which drives fusion of the two opposing bilayers1,2.SNAP-25 is the target of several botulinum neurotoxin (BoNT) types: type A, C and E. The neurotoxins cause specific proteolytic degradation of the SNAP-25 protein, which in turn causes SNARE complex disruption and inhibition of neurotransmitter release3.

Alternative names: SNAP-25, Synaptosomal-associated protein 25 kD

Gene ID: 25012

NCBI Accession: NM_130811

UniProt: P60881

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport