Syntaxin 4 antibody (Intracellular, N-Term)

Catalog No. ABIN7043796

The Rabbit Polyclonal anti-Syntaxin 4 antibody (ABIN7043796) specifically detects Syntaxin 4 in WB, IP and IHC. The antibody is reactive with Rat samples.

Quick Overview for Syntaxin 4 antibody (Intracellular, N-Term) (ABIN7043796)

Target: Syntaxin 4 (STX4)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syntaxin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Product Details anti-Syntaxin 4 Antibody

Binding Specificity: AA 2-23, Intracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Syntaxin 4

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 17,22 amino acid residues identical

Characteristics: Anti-Syntaxin 4 Antibody is directed against an epitope of rat Syntaxin 4. Anti-Syntaxin 4 Antibody (ABIN7043796, ABIN7044650 and ABIN7044651) can be used in western blot and immunohistochemical applications. It was designed to recognize Syntaxin 4 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: RDRTHELRQGDNISDDEDEVRV(C), corresponding to residues 2-23 of rat syntaxin 4

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237347)

Blocking Peptide: (ABIN7237347)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Syntaxin 4

Target: Syntaxin 4 (STX4)

Alternative Name: STX4

Background:

Synonyms: STX4, Renal carcinoma antigen NY-REN-31, P35-2

Description: Syntaxin 4 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.Syntaxin 4, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.Syntaxin 4 functions as a plasma membrane protein t-SNARE and has been shown to have an important role in pancreatic b-cell insulin secretion and in insulin-stimulated glucose uptake in skeletal muscle and adipose tissue by means of translocation of the glucose transporter GLUT4 from an intracellular compartment to the plasma membrane3,4.

Gene ID: 81803

NCBI Accession: NM_004604

UniProt: Q08850

Pathways: Synaptic Vesicle Exocytosis