SNCA antibody (C-Term, Intracellular)

Catalog No. ABIN7887056

The Rabbit Polyclonal anti-SNCA antibody (ABIN7887056) specifically detects SNCA in WB. The antibody is reactive with Rat samples.

Quick Overview for SNCA antibody (C-Term, Intracellular) (ABIN7887056)

Target: SNCA (Synuclein, alpha (SNCA))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNCA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SNCA Antibody

Binding Specificity: AA 97-110, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to α-Synuclein

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 11,14 amino acid residues identical

Characteristics: Anti-Alpha-Synuclein Antibody (ABIN7043799, ABIN7045180 and ABIN7045181) is a highly specific antibody directed against an epitope of the rat protein. It can be used in western blot analysis. It has been designed to recognize α-Synuclein from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KDQMGKGEEGYPQE, corresponding to amino acid residues 97-110 of rat alpha-synuclein

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237358)

Blocking Peptide: (ABIN7237358)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SNCA

Target: SNCA (Synuclein, alpha (SNCA))

Alternative Name: SNCA

Background:

Synonyms: α-Synuclein, Non-A4 component of amyloid precursor, Parkinson disease (autosomal dominant, Lewy body) 4, PARK1, PARK4, NACP, SNCA

Description: α-Synuclein belongs to the Synuclein family of proteins containing three other members (β-synuclein, γ-synuclein and synoretnin). α-Synuclein is a highly conserved acidic protein that is concentrated mainly in presynaptic axon terminals.The gene encoding α-synuclein is known as SNCA or PARK1. α-Synuclein, together with other members of its family, lacks typical secondary structure. In its primary structure, α-synuclein is characterized by seven imperfect 11-residue repeat sequences that are predicted to form five amphipatic helices on the amino terminal half. The acidic, glutamate rich carboxy-terminal region remains unstructured even in the presence of membranes. α-Synuclein helices 1-4 are predicted to associate with lipid vesicles while helix 5 would be responsible for protein-to-protein interaction. The protein interacts and with DAT, the dopamine transporter, and thus is involved in dopamine signaling. Under pathological conditions, α-synuclein can undergo conformational changes leading to protein aggregation and deposition.α-Synuclein shares structural homology with the 14-3-3 family of chaperon proteins and its expression is upregulated during periods of accelerated neuronal activity and plasticity thus suggesting it might be functioning as a molecular chaperon capable of binding to other intercellular proteins.α-Synuclein has been implicated in the pathophysiology of Parkinson's disease (PD) and Lewy body dementia (DLB). Mutations A30P and A3T are associated with PD while mutation E46K has been linked to DLB. These mutated α-synucleins form fibrils more rapidly than the wild-type protein in aqueous solution1. α-Synucleins are the major components of Lewy bodies and Lewy neurites in both diseases2.

Gene ID: 29219

NCBI Accession: NM_000345

UniProt: P37377

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity