TBXA2R antibody (AA 225-239)

Catalog No. ABIN7043809

Product Details anti-TBXA2R Antibody

Target: TBXA2R (Thromboxane A2 Receptor (TBXA2R))

Binding Specificity: AA 225-239

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBXA2R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Thromboxane A2 Receptor

Specificity: 3rd intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - 14,15 amino acid residues identical, human - 12, mouse - 13

Characteristics: Anti-Thromboxane A2 Receptor Antibody (ABIN7043809, ABIN7045124 and ABIN7045125)) is a highly specific antibody directed against an epitope of the rat TBXA2R. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TBXA2R from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HAREATQRPRD(S)EVE, corresponding to amino acid residues 225-239 of rat TBXA2R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-PR069

Blocking Peptide: BLP-PR069

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TBXA2R

Target: TBXA2R (Thromboxane A2 Receptor (TBXA2R))

Alternative Name: TBXA2R (TBXA2R Products)

Background: Prostanoid TP receptor, TXA2-R, TBXA2R,Thromboxane A2 (TxA2) is a prostanoid that is synthesized from arachidonic acid and plays an important role in haemostasis, a process regulating platelet aggregation and vascular tone. Thromboxane A2 binds to the thromboxane A2 receptor (TBXA2R) to induce strong vasoconstriction in renal arteries.Thromboxane A2 receptor is a seven-transmembrane G-protein coupled receptor localized on the cell membrane and in intracellular compartments. TBXA2R is expressed in the central nervous system in several types of cells, such as microglia, oligodendrocytes and astrocytes. Imbalances in the levels of the receptor is widely implicated in several cardiovascular, pulmonary, renal and prostate pathologies1-3.Thromboxane A2 receptor mediates vasoconstriction and ischemia and its activation is implicated in atherosclerosis and inflammation. Activation of the receptor induces endothelial cell apoptosis by inhibiting Akt phosphorylation and also inhibits endothelial cell migration and angiogenesis by decreasing Akt and eNOS phosphorylation1-3.

Alternative names: Thromboxane A2 Receptor, Prostanoid TP receptor, TXA2-R, TBXA2R

Gene ID: 24816

NCBI Accession: NM_001060

UniProt: P34978