TBXA2R antibody (3rd Intracellular Loop)

Catalog No. ABIN7043809

This Rabbit Polyclonal antibody specifically detects TBXA2R in WB, IHC and IF. It exhibits reactivity toward Rat.

Quick Overview for TBXA2R antibody (3rd Intracellular Loop) (ABIN7043809)

Target: TBXA2R (Thromboxane A2 Receptor (TBXA2R))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBXA2R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TBXA2R Antibody

Binding Specificity: 3rd Intracellular Loop, AA 225-239

Purpose: A Rabbit Polyclonal Antibody to Thromboxane A2 Receptor

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - 14,15 amino acid residues identical, human - 12, mouse - 13

Characteristics: Anti-Thromboxane A2 Receptor Antibody (ABIN7043809, ABIN7045124 and ABIN7045125) is a highly specific antibody directed against an epitope of the rat TBXA2R. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TBXA2R from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HAREATQRPRD(S)EVE, corresponding to amino acid residues 225-239 of rat TBXA2R

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237385)

Blocking Peptide: (ABIN7237385)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for TBXA2R

Target: TBXA2R (Thromboxane A2 Receptor (TBXA2R))

Alternative Name: TBXA2R

Background:

Synonyms: Prostanoid TP receptor, TXA2-R, TBXA2R

Description: Thromboxane A2 (TxA2) is a prostanoid that is synthesized from arachidonic acid and plays an important role in haemostasis, a process regulating platelet aggregation and vascular tone. Thromboxane A2 binds to the thromboxane A2 receptor (TBXA2R) to induce strong vasoconstriction in renal arteries.Thromboxane A2 receptor is a seven-transmembrane G-protein coupled receptor localized on the cell membrane and in intracellular compartments. TBXA2R is expressed in the central nervous system in several types of cells, such as microglia, oligodendrocytes and astrocytes. Imbalances in the levels of the receptor is widely implicated in several cardiovascular, pulmonary, renal and prostate pathologies1-3.Thromboxane A2 receptor mediates vasoconstriction and ischemia and its activation is implicated in atherosclerosis and inflammation. Activation of the receptor induces endothelial cell apoptosis by inhibiting Akt phosphorylation and also inhibits endothelial cell migration and angiogenesis by decreasing Akt and eNOS phosphorylation1-3.

Gene ID: 24816

NCBI Accession: NM_001060

UniProt: P34978