TAAR1 antibody (Intracellular)

Catalog No. ABIN7043812

Product Details anti-TAAR1 Antibody

Target: TAAR1 (Trace Amine Associated Receptor 1 (TAAR1))

Binding Specificity: AA 313-325, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAAR1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Trace Amine Receptor 1

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - 12,13 amino acid residues identical

Characteristics: Anti-Trace Amine Receptor 1 (TAAR1) Antibody is directed against an epitope of the rat Trace amine-associated receptor 1. Anti-Trace Amine Receptor 1 (TAAR1) Antibody (ABIN7043812, ABIN7045324 and ABIN7045325) can be used in western blot analysis. It has been designed to recognize TAAR1 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KMVLFGKIFQKDS, corresponding to amino acid residues 313-325 of rat TAAR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237397)

Blocking Peptide: (ABIN7237397)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TAAR1

Target: TAAR1 (Trace Amine Associated Receptor 1 (TAAR1))

Alternative Name: TAAR1 (TAAR1 Products)

Background: Trace amine-associated receptor 1, TaR-1, TA1, TRAR1,Trace amines are compounds, such as p-tyramine and tryptamine, found in the body in trace amounts. These substances were believed to mainly modulate aminergic pathways. However, they were found to activate Trace amino associated receptors (TAARs), members of the G-protein coupled receptor (GPCRs) superfamily1.Trace amine receptor 1 (TAAR-1) is part of the Rhodopsin family of GPCRs. Five other putative genes are thought to encode TAAR receptors. This receptor has a seven transmembrane domain with short extracellular N- and intracellular C- terminal domains.TAAR-1 couples Gs, thus leading to an increase in intracellular cAMP levels. Its signaling might be enhanced by monoamine transporters, and activation of TAAR-1 may also affect monoamine transport function. The receptor also inhibits PKA and PKC mediated dopamine uptake and PKC mediated dopamine efflux2. It is also possible that it forms heterodimers with dopamine receptors, thus influencing dopamine transmission3.In humans, this receptor is mainly expressed in the stomach but is also detected in the lungs, small intestine and many cerebral and cerebellar tissues including the amygdala, hypothalamus and others4.In experiments conducted in mice it was shown that TAAR-1 knockout mice display increased stimulation effects of d-amphetamine, greater levels of extracellular monoamine and hyperactivity. These effects are related to clinical conditions such as ADHD, psychosis and other psychiatric problems5. Additional studies on TAAR family receptors may be helpful in finding cutting edge treatment.

Alternative names: Trace Amine Receptor 1 (TAAR1), Trace amine-associated receptor 1, TaR-1, TA1, TRAR1

Gene ID: 113914

NCBI Accession: NM_138327

UniProt: Q923Y9