TRPM6 antibody (Extracellular)

Catalog No. ABIN7043835

The Rabbit Polyclonal anti-TRPM6 antibody (ABIN7043835) specifically detects TRPM6 in IHC, WB, FACS and LCI. The antibody is reactive with Mouse samples.

Quick Overview for TRPM6 antibody (Extracellular) (ABIN7043835)

Target: TRPM6 (Transient Receptor Potential Cation Channel, Subfamily M, Member 6 (TRPM6))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPM6 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-TRPM6 Antibody

Binding Specificity: AA 802-815, Extracellular

Purpose: A Rabbit Polyclonal Antibody to TRPM6 Channel

Specificity: 1st extracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: 14 amino acid residues identical,Rat - 13, human - 11

Characteristics: Anti-TRPM6 (extracellular) Antibody (ABIN7043835, ABIN7044012 and ABIN7044013) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TRPM6 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CVKDYDLERGPDEK, corresponding to amino acid residues 802-815 of mouse TRPM6

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC

Negative Control: (ABIN7237463)

Blocking Peptide: (ABIN7237463)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRPM6

Target: TRPM6 (Transient Receptor Potential Cation Channel, Subfamily M, Member 6 (TRPM6))

Alternative Name: TRPM6

Background: Transient receptor potential cation channel subfamily M member 6, Channel kinase 2, CHAK2, Melastatin-related TRP cation channel 6,TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the six TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology, TRPC (canonical), TRPV (vanilloid) and TRPM (melastatin). To date, three extra subfamilies are also considered to belong to the TRP family, the TRPA, TRPML, and the TRPP.1-4The TRPM subfamily consists of eight members, TRPM1 to TRPM8, which also can be further subdivided into four subgroups based on their sequence homology: (1)TRPM1 and TRPM3 (2) TRPM6 and TRPM7 (3) TRPM4 and TRPM5 (4) TRPM2 and TRPM8.5

Alternative names: TRPM6, Transient receptor potential cation channel subfamily M member 6, Channel kinase 2, CHAK2, Melastatin-related TRP cation channel 6

Gene ID: 225997

NCBI Accession: NM_017662

UniProt: Q8CIR4