TMEM38A antibody (Intracellular)

Catalog No. ABIN7043861

Product Details anti-TMEM38A Antibody

Target: TMEM38A (Transmembrane Protein 38A (TMEM38A))

Binding Specificity: AA 259-274, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMEM38A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to TRIC-A Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - will not recognize human TRIC-A

Characteristics: Anti-TRIC-A (TMEM38A) Antibody (ABIN7043861 and ABIN7045315)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRIC-A from rat and mouse samples. The antibody is not suited to recognize TRIC-A from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DNHGAPHGMGLGTQHS, corresponding to amino acid residues 259-274 of rat TRIC-A

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-TC002

Blocking Peptide: BLP-TC002

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TMEM38A

Target: TMEM38A (Transmembrane Protein 38A (TMEM38A))

Alternative Name: TMEM38A (TMEM38A Products)

Background: Trimeric intracellular cation channel type A, 27 kDa sarcoplasmic reticulum protein, Transmembrane protein 38A,Intracellular Ca2+ levels are important in proper cellular functions and have prominent roles in various cell signaling pathways and are crucial for muscle contractions. Indeed, an important step leading to muscle contraction is the massive release of Ca2+ ions from the endoplasmic/sarcoplasmic reticulum (ER/SR) to the cytosol. A battery of results suggest that specific K+ channels are important to counteract the Ca2+ outflow in order to neutralize the negative potential created by the movement of Ca2+ ions. It is believed that TRIC channels are responsible for neutralizing this negative potential1,2.Trimeric intracellular cation-specific (TRIC) channels are critical for proper management of intracellular stores. TRIC-A and TRIC-B both belong to this family and are both permeable to monovalent ions with a preference for K+ 3. Both channels are localized to the ER/SR membrane. Each TRIC subunit contains three transmembrane domains, a cytoplasmic C-terminus and a luminal N-terminus. Functional entities are formed by homotrimerizarion4. The activity of TRIC-A is regulated by voltage whereas that of TRIC-B can be regulated by different mechanisms3.Knockout studies of these channels have shown that TRIC-A knockout mice are viable while those of TRIC-B die at the neonatal stage5.TRIC-A is mostly expressed in excitable tissues like the brain and muscle while TRIC-B is ubiquitously expressed.

Alternative names: TRIC-A (TMEM38A), Trimeric intracellular cation channel type A, 27 kDa sarcoplasmic reticulum protein, Transmembrane protein 38A

Gene ID: 306327

NCBI Accession: NM_024074

UniProt: A6ZIQ8