TMEM66 antibody (Intracellular) (Atto 594)

Catalog No. ABIN7043864

The Rabbit Polyclonal anti-TMEM66 antibody has been validated for IF and IHC. It is suitable to detect TMEM66 in samples from Rat.

Quick Overview for TMEM66 antibody (Intracellular) (Atto 594) (ABIN7043864)

Target: TMEM66 (Transmembrane Protein 66 (TMEM66))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMEM66 antibody is conjugated to Atto 594

Application: Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-TMEM66 Antibody (Atto 594)

Binding Specificity: AA 141-155, Intracellular

Purpose: A Rabbit Polyclonal Antibody to TMEM66 Conjugated to the Fluorescent Dye ATTO-594

Specificity: Intracellular, lumen.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 15 amino acid residues identical,Mouse - 13, human - 11

Characteristics: Anti-TMEM66 Antibody (ABIN7043863, ABIN7044034 and ABIN7044035) is a highly specific antibody directed against an epitope of rat transmembrane protein 66. The antibody can be used in western blot analysis. It has been designed to recognize TMEM66 from human, rat, and mouse samples. \nAnti-TMEM66-ATTO Fluor-594 Antibody (ABIN7043864) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-TMEM66-ATTO Fluor-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ELGLSKLKESGKHQS, corresponding to amino acid residues 141-155 of rat SARAF

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7237528)

Blocking Peptide: (ABIN7237528)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TMEM66

Target: TMEM66 (Transmembrane Protein 66 (TMEM66))

Alternative Name: SARAF

Background: Store-operated calcium entry-associated regulatory factor, SOCE-associated regulatory factor, SARAF, Transmembrane protein 66,TMEM66 (Transmembrane protein 66, also known as SOCE-associated regulatory factor, SARAF) is a regulatory protein of cellular Ca2+ homeostasis, encoded by the TMEM66 gene. TMEM66 is an ER resident protein, which responds to cytosolic Ca2+ elevation after ER Ca2+ refilling.TMEM66 is highly conserved in vertebrates. In mammals it is ubiquitously expressed with high transcript levels in the immune and neuronal tissues. The TMEM66 structure contains validated signal peptide and a putative single membrane spanning domain, a serine-proline rich domain and arginine rich regions followed by a cluster of basic residues at its C-terminal tail1.Store-operated Ca2+ entry (SOCE) is an important Ca2+ influx pathway across plasma membranes in many non-excitable cells. In this pathway, intracellular Ca2+ release through IP3 receptors stimulated by a variety of agonists, results in reduction of Ca2+ concentration in the lumen of the endoplasmic or sarcoplasmic reticulum. SARAF negatively regulates store operated calcium entry into cells and protects cells from calcium overfilling1,2.STIM, Ca2+ sensor, and Orai, the channel pore forming subunit proteins have been identified as the essential components enabling the reconstitution of Ca2+ release-activated Ca2+ channels that mediate SOCE. Knock-out of Orai1 or STIM1 in mice and mutations evoke SOCE in lymphocytes and other cells2,3.Findings show that dysregulation of Ca2+ homeostasis is associated with a plethora of pathological conditions including neurodegenerative diseases, skeletal, muscular and cardiovascular disorders2. Inherited defects in SOCE due to mutations in genes of the Ca2+ release-activated Ca2+ (CRAC) channel complex causes patients to suffer from a severe form of immunodeficiency that is due to defects in the function of T cells, NK cells and potentially other immune cells3.

Alternative names: TMEM66, Store-operated calcium entry-associated regulatory factor, SOCE-associated regulatory factor, SARAF, Transmembrane protein 66

Gene ID: 290796

NCBI Accession: NM_016127

UniProt: Q6AYN2