VIPR1 antibody (Extracellular, N-Term)

Catalog No. ABIN7043875

This Rabbit Polyclonal antibody specifically detects VIPR1 in WB, IHC, IF, FACS, LCI and IC. It exhibits reactivity toward Human.

Quick Overview for VIPR1 antibody (Extracellular, N-Term) (ABIN7043875)

Target: VIPR1 (Vasoactive Intestinal Peptide Receptor 1 (VIPR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VIPR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Product Details anti-VIPR1 Antibody

Binding Specificity: AA 52-65, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to VPAC1 Receptor

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human - 13,14 amino acid residues identical, rat,mouse - 12

Characteristics: Anti-VPAC1 (VIPR1) (extracellular) Antibody (ABIN7043875, ABIN7045329 and ABIN7045330) is a highly specific antibody directed against an epitope of human VIP and PACAP receptor 1. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize VPAC1 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EEAQLENETIG(S)SK, corresponding to amino acid residues 52-65 of human VPAC1

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: 2.5 mg per reaction

ICC: 1:25

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IFC

Negative Control: (ABIN7237555)

Blocking Peptide: (ABIN7237555)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for VIPR1

Target: VIPR1 (Vasoactive Intestinal Peptide Receptor 1 (VIPR1))

Alternative Name: VIPR1

Background:

Synonyms: VIP and PACAP receptor 1, Vasoactive intestinal polypeptide receptor 1, Pituitary adenylate cyclase-activating polypeptide type II receptor, PACAP type II receptor, PACAP-R2

Description: Vasointestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP) belong to the glucagon hormone superfamily, which includes secretin, growth hormone-releasing hormone (GHRH), glucagon, glucagon-like peptides 1 and 2 (GLP-1 and GLP-2), peptide histidine methionine (PHM), and glucose-dependent insulinotropic polypeptide (GIP)1. PACAP and VIP effects have been described in the digestive tract, cardiovascular system, airways, reproductive system, immune system, endocrine glands, and brain2. VIP and PACAP share a common G-protein coupled receptor, VPAC13.VPAC1 is a membrane-associated protein and shares significant homology with members of the G-protein coupled class B receptor family, the most important of which is the presence of large N-terminal extracellular domains which contain 10 highly conserved amino acids including six cysteines, putative N-terminal leader sequences and several potential N-glycosylation sites4. In the CNS, VPAC1 receptors are abundantly localized in piriform cortex, cerebral cortex, suprachiasmatic nucleus, hippocampus, and pineal gland5. In peripheral tissues, VPAC1 receptors have been found in breast, kidney, liver, lung, prostate, spleen, and mucosa of the gastrointestinal tract6. VPAC1 mediates a large array of VIP and PACAP actions on exocrine secretion, hormones release, muscle relaxation, metabolism, fetus growth, tumor cells and embryonic brain development7.

Gene ID: 7433

NCBI Accession: NM_004624

UniProt: P32241