Recombinant CD28 antibody

Catalog No. ABIN7072234

This Mouse Monoclonal antibody specifically detects CD28 in FACS, IF, IHC, CoSt and Agon. It exhibits reactivity toward Rat.

Quick Overview for Recombinant CD28 antibody (ABIN7072234)

Target: CD28

Antibody Type: Recombinant Antibody

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Co-Stimulation (CoSt), Agonist (Agon)

Clone: JJ316

Product Details anti-CD28 Antibody

Purpose: Anti-CD28 [JJ316], Mouse IgG1, kappa

Specificity:

Recognises rat CD28 and can trigger T-cell proliferation in vitro and in vivo.

Application Notes original Clone: This antibody binds rat CD28 and can convey as a co-stimulatory signal. At 100-1000-fold higher concentration than required for co-stimulation JJ316 (unlike the clone JJ319 marketed by competitors (see Tacke et al. 1997)) can induce proliferation of all T-cell subsets without TCR engagement.

Purification: Protein A affinity purified

Immunogen: Made by immunizing BALB/c mice with rat CD28 cDNA-transfected A20/J cells.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: TP44, T-cell-specific surface glycoprotein CD28

UniProt: P31042

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response