Recombinant Ly6g antibody

Catalog No. ABIN7072246

This Rabbit Chimeric antibody specifically detects Ly6g in FACS and Dep. It exhibits reactivity toward Mouse.

Quick Overview for Recombinant Ly6g antibody (ABIN7072246)

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This Ly6g antibody is un-conjugated

Application: Flow Cytometry (FACS), Depletion (Dep)

Clone: 1A8

Product Details anti-Ly6g Antibody

Purpose: Anti-Ly6G [1A8], Rabbit IgG, kappa

Specificity: Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells

Isotype: IgG kappa

Application Details

Application Notes: Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion < a href="http://www.ncbi.nlm.nih.gov/pubmed/17884993" target="_blank"> in vivo. It may also be used as a negative selection marker for bone-marrow derived macrophages, which should be Ly6G(-) and F4/80 (+) < a href="http://absoluteantibody.com/ab/anti-f480-cia3-1-recombinant-version/" target="_blank"> (see our F4/80 range of antibodies) . The mouse IgG2a version of the antibody, recombinantly produced by Absolute Antibody, could deplete neutrophils in mice more efficiently than the rat version of the antibody. Further, this version increased depleting effector function, and prevented the production of anti-rat antibodies (Olofsen et al., 2022, PMID: 36359801).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Ly6g

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Alternative Name: Ly6G

Background: LGR-1 , y-6G, GR1, Lymphocyte antigen 6G

UniProt: P35461