Recombinant Rhodopsin antibody

Catalog No. ABIN7072289

The Mouse Chimeric anti-Rhodopsin antibody has been validated for WB, IHC, ELISA, IP, IF and Lbl. It is suitable to detect Rhodopsin in samples from Human, Cow, Rat, Mouse, Zebrafish (Danio rerio) and Amphibian.

Quick Overview for Recombinant Rhodopsin antibody (ABIN7072289)

Target: Rhodopsin (RHO)

Antibody Type: Recombinant Antibody

Reactivity: Human, Cow, Rat, Mouse, Zebrafish (Danio rerio), Amphibian

Host: Mouse

Clonality: Chimeric

Conjugate: This Rhodopsin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Labeling (Lbl)

Clone: Rho 1D4

Product Details anti-Rhodopsin Antibody

Purpose: Anti-Rhodopsin [Rho 1D4], Mouse IgG1, kappa

Specificity: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.

Cross-Reactivity: Amphibian, Cow, Human, Rat, Zebrafish (Danio rerio)

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: Bleached bovine rod outer segment (ROS).

Isotype: IgG1 kappa

Application Details

Application Notes: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein-coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag.

Comment:

This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Rhodopsin

Target: Rhodopsin (RHO)

Alternative Name: Rhodopsin

Target Type: Chemical

Background: Rho-tag, Visual Purple

UniProt: P08100

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction