Recombinant CD44 antibody

Catalog No. ABIN7072357

This Rabbit Chimeric antibody specifically detects CD44 in FACS, WB, IHC, ELISA, IF, IP, ICC, Neut and BP. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Cow, Sheep, Goat and Primate.

Quick Overview for Recombinant CD44 antibody (ABIN7072357)

Target: CD44

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Sheep, Goat, Primate

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Neutralization (Neut), Blocking Peptide (BP)

Clone: Hermes-1

Product Details anti-CD44 Antibody

Purpose: Anti-CD44 [Hermes-1], Rabbit IgG, kappa

Specificity: This antibody is specific for CD44 (gp90 epitope cluster I), which is selectively expressed on normal or transformed lymphoid cells that are able to recognize and bind to high endothelial venules (specialized vessels that mediate lymphocyte exit from the blood into lymphoid organs). Although this antibody was raised against human CD44, it cross-reacts with murine, rat, rabbit, primate, sheep, bovine and goat CD44.

Cross-Reactivity: Cow, Goat, Mouse, Primate, Rabbit, Rat, Sheep

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was produced by immunizing a Fischer rat with human tonsil lymphocytes (10^7cells intraperitoneally in Freund's complete adjuvant). A boost was given 2 weeks later (intravascularly in saline).

Isotype: IgG kappa

Application Details

Application Notes: This antibody binds CD44, so can be used to label CD44+ cells in flow cytometry analysis. As CD44 Molecules are used to bind to high endothelial venules (HEV) at the site of lymphoctye entry to lymphoid organs, this antibody can be used to specifically stain HEV-binding cell lines (Jalkanen, 1986). In addition, this antibody blocks the adhesion of the haematopoietic progenitor cell line KG1 to hyaluronic acid (Bendall, 2003), but does not block lymphocyte binding to HEV (Picker, 1989). 7H9D6 partially blocks the binding of this antibody.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, CD44/H-CAM antibody, Hermes 1

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling