Recombinant FCGR1A antibody

Catalog No. ABIN7072384

This Rabbit Chimeric antibody specifically detects FCGR1A in FACS, ELISA and CyTox. It exhibits reactivity toward Human.

Quick Overview for Recombinant FCGR1A antibody (ABIN7072384)

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This FCGR1A antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Cytotoxicity Test (CyTox)

Clone: H22

Product Details anti-FCGR1A Antibody

Purpose: Anti-CD64 [H22], Rabbit IgG, kappa

Specificity: This antibody is specific for human CD64, specifically an epitope outside the Fc binding domain. The binding activity of the VH/VL antibody format was unaffected by the fusion of the m22(scFv) coding regions to the truncated ETA coding sequences.

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A (ETA' ).

Isotype: IgG kappa

Application Details

Application Notes: This antibody binds human CD64 and can be bound to a toxin so that together it displays specific cytotoxicity, and thus can be used to selectively eliminate AML cells, FC analysis indicated that this immunotoxin drove 41 % of primary leukemia cells from a patient with CD64-positive AML into early apoptosis (Tur, 2003). Similarly, in an in vivo mouse model, IHC analysis revealed efficient elimination of human CD64+ tumor cells in mouse organs (Tur, 2011).

Comment:

This is a chimeric rabbit IgG antibody based on the original human IgG1 antibody, created for improved compatibility with existing reagents, assays, and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for FCGR1A

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Alternative Name: CD64

Background: FcγRI, High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, FcRI, Fc-gamma RIA

UniProt: P12314

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process