Recombinant EDAR antibody (C-Term, Extracellular Domain)

Catalog No. ABIN7072395

The Rabbit Chimeric anti-EDAR antibody (Clone EDAR12) (ABIN7072395) specifically detects EDAR in WB, ELISA, SPR and Agon. The antibody is reactive with Human, Mouse, Rat, Chicken and Dog samples.

Quick Overview for Recombinant EDAR antibody (C-Term, Extracellular Domain) (ABIN7072395)

Target: EDAR (Ectodysplasin A Receptor (EDAR))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Chicken, Dog

Host: Rabbit

Clonality: Chimeric

Conjugate: This EDAR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Surface Plasmon Resonance (SPR), Agonist (Agon)

Clone: EDAR12

Product Details anti-EDAR Antibody

Binding Specificity: C-Term, Extracellular Domain

Purpose: Anti-EDAR [EDAR12], Rabbit IgG, kappa

Specificity:

EDAR12 recognises and binds to CRD1 of the extracellular domain of mouse EDAR. EDAR3 cross-reacts with EDAR derived from human, dog, rat and chicken when EDAR is fused to the glycosylphosphatidylinositol anchor of TRAILR3. EDAR is the receptor for the TNF family ligand EDA1, which is a type II transmembrane protein possessing a collagen-like domain and a C-terminal TNF-homology domain. EDAR is important for the correct development of skin appendages including hair, teeth and eccrine sweat glands. LoF mutations in the Eda gene is known to cause XLHED (X-linked hypohidrotic ectodermal dysplasia), and results in abnormal development.

Application Notes original Clone: EDAR12 can be used in surrogate reporter assay in which Fas-sensitive cells were transfected with a construct formed by fusing the ectodomain of mouse or human EDAR to the intracellular domain of Fas. Binding of EDAR12 resulted in the induction of apoptosis in these cells, confirming the agonisitc activity of the antibody. (EC50 (dose required to kill half of EDAR:Fas-expressing Jurkat cells) hEDAR:Fas ~ 5 ng/mL, EC50 mEDAR:Fas ~ 10 ng/mL - both were less active than DEA1-Fc). Tail-hair formation was also rescued after administration of EDAR3 to newborn EDA-deficient Tabby mice, with 0.35 mg/kg required for half-maximal tail-hair reversion. EDAR123 can also be used in ELISA and as a Fab fragment (generated by ficin digestion) in SPR experiments. EDAR can also be recognized by EDAR12 under both reducing (+DTT) and non-reducing conditions.

Cross-Reactivity: Chicken, Dog, Mouse, Rat

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogen: EDAR12 was prepared by immunixzing female OVE1B mice (with the Edar gene deleted) subcutaneously with mouse EDAR-Fc and positive hybridoma clones were screened for binding mEDAR by ELISA.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for EDAR

Target: EDAR (Ectodysplasin A Receptor (EDAR))

Alternative Name: EDAR

Background: Ectodysplasin A receptor, Tumor necrosis factor receptor superfamily member EDAR, Anhidrotic ectodysplasin receptor 1, Downless, Ectodermal dysplasia receptor, Ectodysplasin-A receptor

UniProt: Q9R187

Pathways: Tube Formation, Ubiquitin Proteasome Pathway