Recombinant LTA antibody

Catalog No. ABIN7072400

The Mouse Monoclonal anti-LTA antibody has been validated for ELISA, FACS, Neut and SPR. It is suitable to detect LTA in samples from Human.

Quick Overview for Recombinant LTA antibody (ABIN7072400)

Target: LTA (Lymphotoxin-alpha (LTA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LTA antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Neutralization (Neut), Surface Plasmon Resonance (SPR)

Clone: 3F12-2D3

Product Details anti-LTA Antibody

Purpose: Anti-Lymphotoxin alpha [3F12.2D3], Mouse IgG2b, kappa

Specificity:  3F12 binds to human LTα3 and LTαβ (with a KD of ~0.3 nM, determined by BIACORE, ~ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTα belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTα3), or is expressed on the cell surface in complex with LTβ. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTα3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: 3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: The properties of the chimieric 3F12 is equivalent to that of the original 3F12 mAb. 3F12 is able to bind to Th2 cells and additionally is can functionally block human LTα3 versus an isotype control (trastuzumab) - LTα3-induced ICAM upregulation is blocked on HUVEC (Human Umbilical Endothelial Cells) (IC50 0.04 nM) and LTα3-induced IL-8 is blocked in A549 cells. The antibody has also been used in ELISA studies to detect LT-alpha and to measure binding.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for LTA

Target: LTA (Lymphotoxin-alpha (LTA))

Alternative Name: Lymphotoxin alpha

Target Type: Chemical

Background: LTα3 chimeric 3F12,LT, LTα, Tumor necrosis factor beta, Tumor necrosis factor β, Tumor necrosis factor-beta, tumor necrosis factor-β, TNF beta, TNF-beta, TNF β, TNF-β, Lymphotoxin-alpha, Lymphotoxin alpha

UniProt: P01374

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process