Recombinant BTLA antibody

Catalog No. ABIN7072471

This Hamster Chimeric antibody specifically detects BTLA in WB, FACS, IP, Func and BP. It exhibits reactivity toward Mouse.

Quick Overview for Recombinant BTLA antibody (ABIN7072471)

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Hamster

Clonality: Chimeric

Conjugate: This BTLA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Blocking Peptide (BP)

Clone: HMBT-6B2

Product Details anti-BTLA Antibody

Purpose: Anti-BTLA [HMBT-6B2], Hamster (Armenian) IgG, kappa

Specificity: This antibody is specific for B and T lymphocyte attenuator (BTLA), which functions as a negative regulator of T cell activation and proliferation, and attenuates B cell proliferation upon associating with its known ligand, herpes virus entry mediator (HVEM).

Characteristics:

Original Species of Ab: Hamster, Armenian

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Armenian hamsters with mouse BTLA:Fc fusion protein.

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been used in immunoblot analyses to confirm that B and T lymphocyte attenuator (BTLA) regulates T cell activation through interaction with herpesvirus entry mediator (HVEM) (Sedy et al, 2005), as well as in FACS and yeast display technology to demonstrate that BTLA exhibits structural and expression polymorphisms and is highly induced in anergic CD4+ T Cells (Hurchla et al, 2005). In a mouse conjunctivitis model, treatment with this antibody during the induction phase has been shown to decrease B-cell population, upregulate Th2 cytokine production and increase the conjunctival eosinophile numbers (Ishida et al, 2012). However, treatment with this antibody during the effector phase does not affect the development of experimental conjunctivitis (Ishida et al, 2012).

Comment:

This chimeric hamster (Armenian) antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for BTLA

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Alternative Name: BTLA

Background: CD272, B and T lymphocyte attenuator, B- and T-lymphocyte attenuator, B and T lymphocyte associated protein, B- and T-lymphocyte-associated protein, BTLA1, FLJ16065, MGC129743

UniProt: Q7TSA3

Pathways: Cancer Immune Checkpoints