Recombinant CD20 antibody

Catalog No. ABIN7072520

The Rabbit Chimeric anti-CD20 antibody has been validated for FACS, WB, IF, Func and Dep. It is suitable to detect CD20 in samples from Mouse.

Quick Overview for Recombinant CD20 antibody (ABIN7072520)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD20 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Functional Studies (Func), Depletion (Dep)

Clone: 18B12

Product Details anti-CD20 Antibody

Purpose: Anti-CD20 [18B12], Rabbit IgG, kappa

Specificity: This antibody is specific for murine CD20, which is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells. Low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells. Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was generated by immunizing CD20-/- mice with CD20-transfected cell lines (300.18 and 70Z3) and CD20-peptide-keyhole limpet hemocyanin conjugate.

Isotype: IgG kappa

Application Details

Application Notes: In the original publication, this antibody was used in FACS and in vivo functional assays to examine how B cell depletion affects autoimmunity (Ahuja et al., 2007). In other studies, this antibody has been used mainly in FACS, immunofluorescence, and various in vitro and in vivo functional experiments. More recent works employing this antibody have shown, for instance, that enhanced phagocytosis of circulating B cells by Kupffer cells represents an important in vivo mechanism for improved activity of glycoengineered anti-CD20 mAbs (Grandjean et al., 2016), that B lymphocytes limit senescence-driven fibrosis resolution and favor hepatocarcinogenesis in mouse liver injury (Faggioli et al., 2017), and that B cells inhibit the antitumor immunity against an established murine fibrosarcoma (Maglioco et al., 2017).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: CD20

Background: Ly-44, B-cell differentiation antigen Ly-44, B-lymphocyte antigen CD20, CD20, CD20 antigen, Lymphocyte antigen 44, Membrane-spanning 4-domains subfamily A member 1, membrane-spanning 4-domains, subfamily A, member 2

UniProt: P19437