Recombinant CEA antibody

Catalog No. ABIN7072526

The Rabbit Chimeric anti-CEA antibody is suitable to detect CEA in samples from Human. It has been validated for IHC and Lbl.

Quick Overview for Recombinant CEA antibody (ABIN7072526)

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CEA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Labeling (Lbl)

Clone: A5B7

Product Details anti-CEA Antibody

Purpose: Anti-carcinoembryonic antigen [A5B7], Rabbit IgG, kappa

Specificity:

This antibody is specific for human CEA.

Application Notes original Clone: In IHC analysis, this CEA-specific antibody stains sections of colon tumour, with high levels of cell surface and cytoplasmic staining of malignant glands, and a more intense reaction in necrotic debris (Harwood et al, 1986). It does not cross-react with PMNs, erythrocytes or liver tissue. In an mouse model transplanted with the human colonic tumour MaWi, the weight of the xenograft has a profound effect on the uptake of a radiolabelled version of antibody (Pedley et al, 1987). In a xenograft tumour mouse model based on the adenocarcinoma cell line LS174T, both this antibody and its FAb have a significant effect on tumour growth when administered as radioimmunotherapeutic agents (Pedley et al, 1993). This antibody has also been shown to function as a radioimmunotherapy agent in a Phase I trial in gastrointestinal adenocarcinomas (Meyer et al, 2009), and has been shown in early clinical trials to target an enzyme capable of cleaving an inactive prodrug to release an active, cytotoxic drug to colorectal cancers (Bagshawe et al, 1991).

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Purification: Purified antibody.

Immunogen: This antibody was raised by immunising mice with both carcinoembryonic antigen (CEA) purified from the metastases of colonic tumours, and heat-treated CEA.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CEA

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Alternative Name: carcinoembryonic antigen

Background: CEA, CD66, Q7KZ28, Q13985, Q14081

UniProt: Q13982