Recombinant Fc epsilon RI/FCER1A antibody

Catalog No. ABIN7072532

The Rabbit Chimeric anti-Fc epsilon RI/FCER1A antibody has been validated for FACS, IHC, IP and Dep. It is suitable to detect Fc epsilon RI/FCER1A in samples from Mouse.

Quick Overview for Recombinant Fc epsilon RI/FCER1A antibody (ABIN7072532)

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This Fc epsilon RI/FCER1A antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Depletion (Dep)

Clone: Mar-1

Product Details anti-Fc epsilon RI/FCER1A Antibody

Purpose: Anti-FceR1 alpha [Mar-1], Rabbit IgG, lambda

Specificity: This antibody specifically targets the non-signalling alpha chain of the FceR1 complex.

Characteristics:

Original Species of Ab: Hamster, Armenian

Original Format of Ab: IgG

Purification: Protein A affinity purified

Isotype: IgG lambda

Application Details

Application Notes: This antibody has been used in flow cytometric analysis of FceR1 alpha cell surface expression by basophils (Min et al, 2004, Chen et al, 2009), and immunohistochemical analysis of epidermal sheets (Holzmann et al, 2004). It has also been used in immunoprecipitation analysis of cell lysates from lung cDCs and MC/9 mast cells (Grayson et al, 2007). In vitro incubation of basophils with this antibody blocks subsequent MAR-1 staining, but does not activate basophil IL-4 production (Sokol et al, 2008). Intravenous treatment of mice with this antibody results in basophil depletion in peripheral blood, spleen, bone marrow and liver, but not skin or intraperitoneal mast cell depletion (Sokol et al, 2008). Following treatment with this antibody, basophil-depleted mice display impaired Th2 differentiation upon papain immunisation (Sokol et al, 2008). However, MAR-1 treated mice respond normally to high-dose intravenous Ig in both the K/BxN serum transfer arthritis and collagen-induced arthritis models, despite basophil depletion (Campbell et al, 2014).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Armenian Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Fc epsilon RI/FCER1A

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Alternative Name: FceR1 alpha

Background: Fcer1a, Fc epsilon RI alpha, FceRIa, FceRI alpha, FceRI-α, high affinity IgE receptor, High affinity immunoglobulin epsilon receptor subunit alpha,Fce1a, Fc-epsilon RI-alpha, FcERI, IgE Fc receptor subunit alpha

UniProt: P20489

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process