Recombinant MARCO antibody (Extracellular Domain)

Catalog No. ABIN7072581

The Rabbit Chimeric anti-MARCO antibody (Clone PLK1) (ABIN7072581) specifically detects MARCO in WB, IP, FACS, IF and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Recombinant MARCO antibody (Extracellular Domain) (ABIN7072581)

Target: MARCO (Macrophage Receptor with Collagenous Structure (MARCO))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This MARCO antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: PLK1

Product Details anti-MARCO Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-MARCO [PLK1], Rabbit IgG, kappa

Specificity:

This antibody recognises the extracellular domain V between residues 420 and 431 in MARCO both in human and bovine samples.

Application Notes original Clone: The specificity of this antibody was confirmed in an IF experiment where the antibody stained alveolar macrophages for MACRO (Arredouani et al., 2005). Tissue samples prepared in acetone and paraformaldehyde were also successfully immunohistochemically stained (Arredouani et al., 2005, Martens et al., 2006). The antibody was also capable of weak staining of alveolar macrophages in flow cytometry (Palecanda and Kobzik, 2001). In IP experiments, the antibody immunoprecipitates MARCO from lysates obtained from COS cells transfected with human MARCO (Arredouani et al., 2005).

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogen: The antibody was raised by immunising BALB/c mice with human alveolar macrophages (AMs) and subsequent creation of hybridomas.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG3

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for MARCO

Target: MARCO (Macrophage Receptor with Collagenous Structure (MARCO))

Alternative Name: MARCO

Background: SCARA2, macrophage receptor MARCO, macrophage receptor with collagenous structure, scavenger receptor class A member 2, PLK-1, PLK 1

UniProt: Q9UEW3

Pathways: Activation of Innate immune Response